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Obtaining High-Quality Planting Material of Forest Berry Plants by Clonal Micropropagation for Restoration of Cutover Peatlands

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S.S. Makarov, I.B. Kuznetsova, A.I. Chudetsky, S.A. Rodin

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The results of studies on the improvement of technology for producing highquality planting material of half-high blueberry and Arctic bramble by the method of clonal micropropagation are presented in the current paper. Creation of forest berry plantations in peat extraction areas allows reducing environmental damage and significantly increasing the efficiency of the timber industry. In recent decades, there has been an increasing interest in the creation of forest berry plantations on drained and cutover peatlands in Russia and other countries. It is necessary to use varietal planting material for the successful cultivation of forest berry plants on an industrial scale. Clonal micropropagation is the most effective of the vegetative methods for obtaining planting material, which allows receiving a huge amount of healthy planting material all year round in the conditions of a small laboratory area. Chloride-free ecosterilizer and bleaching agent based on sodium hypochlorite “Belizna” with an exposure of 15 and 20 min showed high efficiency in sterilization of explants of half-high blueberry and Arctic bramble. The highest viability of explants of the studied forest berry crops was observed when sterilized with a 0.1 % mercuric chloride solution and 15 min exposure, and its sharp decrease at 20 min exposure. At the stage of micropropagation, with an increase in the concentration of cytokinin 6-BAP from 0.5 to 1.0 mg/L on the nutrient Woody Plant Medium the number of shoots in regenerated plants of half-high blueberry (Northcountry and Northblue cultivars) and Arctic bramble (Anna and Sofia cultivars) increased. The effect of the concentration of IBA-derived auxin on the number and length of roots of regenerated plants was observed at the in vitro rooting stage.


Sergey S. Makarov1, Candidate of Agriculture, Senior Research Scientist;
ResearcherID:AAB-4651-2021, ORCID:
Irina B. Kuznetsova2, Candidate of Agriculture, Assoc. Prof.; ResearcherID: AAB-4568-2021,
Anton I. Chudetsky1, Leading Engineer; ResearcherID: H-1210-2019,
Sergey A. Rodin3, Doctor of Agriculture, Academician of RAS, Deputy Director for Research; ORCID:


1Central-European Forest Experiment Station, ARRISMF, prosp. Mira, 134, Kostroma, 156013, Russian Federation; e-mail:,
2Kostroma State Agricultural Academy, ul. Uchebnyy gorodok, 34, pos. Karavayevo, Kostroma district, Kostroma region, 156530, Russian Federation; e-mail:
3All-Russian Research Institute of Silviculture and Mechanization of Forestry, ul. Institutskaya, 15, Pushkino, Moscow region, 141202, Russian Federation


Arctic bramble, clonal micropropagation, cultivar, cutover peatlands, growth regulator, half-high blueberry, in vitro

For citation

Makarov S.S., Kuznetsova I.B., Chudetsky A.I., Rodin S.A. Obtaining High-Quality Planting Material of Forest Berry Plants by Clonal Micropropagation for Restoration of Cutover Peatlands. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 2, pp. 21–29. DOI: 10.17238/0536-1036-2021-2-21-29


  1. Gladkova L.I. Growing of Blueberry and Cranberry. Moscow, VNIITEISKh Publ., 1974. 64 p.
  2. Koshcheyev A.K., Smirnyakov Yu.I. Wild Berries: A Handbook. Moscow, Ekologiya Publ., 1992. 270 p.
  3. Kuminov E.P. Unconventional Garden Crops. Michurinsk, 1994. 358 p.
  4. Nikolayev I., Nikolayeva Yu. Arctic Bramble – A Forgotten Berry. Nauka i Zhizn’ [Science and Life], 1997, no. 8, pp. 130–131.
  5. Tyak G.V., Makarov S.S., Kalashnikova E.A., Tyak A.V. Reproduction and Cultivation of the Arctic Bramble (Rubus arcticus L.). Plodovodstvo i yagodovodstvo Rossii [Pomiculture and small fruits culture in Russia], 2018, vol. 52, pp. 95–99.
  6. Tyak G.V., Makeev V.A., Kurlovich L.E., Makeeva G.Yu., Tyak A.V. Prospects of Forest Berry Plants Cultivation and Breeding in the Kostroma Region. Proceedings of the Regional Conference “Nature of the Kostroma Region: Modern State and Ecomonitoring”, Kostroma, March 24–25, 2017. Kostroma, 2017, pp. 146–151.
  7. Cherkasov A.F., Mironov K.A., Shutov V.V., Tyak G.V., Verem’yeva S.S., Makeyev V.A., Makeyeva G.Yu., Ryzhova N.V. Non-Timber Forest Resources of the Kostroma Region: Wild Fruits and Berries, Medicinal Plants and Mushrooms: Scientific Monograph. Ed. by Shutov et al. Kostroma, KSTU Publ., 2006. 250 p.
  8. Blueberry Nursery Stock. Commercial Growers Catalog & Price List. Oregon, Fall Creek Farm & Nursery Inc., 2000. 20 p.
  9. Bomser J., Madhavi D.L., Singletary K., Smith M.A.L. In Vitro Anticancer Activity of Fruit Extracts from Vaccinium Species. Planta Medica, 1996, vol. 62(3), pp. 212–216. DOI: 10.1055/s-2006-957862
  10. Hildreth A.C. Propagation of the Lowbush Blueberry. American Society for Horticultural Science, 1929, vol. 26, pp. 91–92.
  11. Jamieson A.R., Nickerson N.L. Field Performance of the Lowbush Blueberry Propagated by Seed, Stem Cuttings and Micropropagation. Acta Horticulturae, 2003, vol. 626, pp. 423–428. DOI: 10.17660/ActaHortic.2003.626.58
  12. Karp K., Starast M., Värnik R. The Arctic Bramble (Rubus arcticus L.) – The Most Profitable Wild Berry in Estonia. Baltic Forestry, 1997, vol. 3, no. 2, pp. 47–52.
  13. Noormets M., Karp K., Paal T. Recultivation of Opencast Peat Pits with Vaccinium Culture in Estonia. WIT Transactions on Ecology and the Environment. Vol. 64: Ecosystems and Sustainable Development IV, 2003, vol. 2, pp. 1005–1014. DOI: 10.2495/ECO030242
  14. Paal T. Cultivation of Vaccinium angustifolium from Seed. Proceedings of the International Conference “Problems of Rational Utilization and Reproduction of Berry Plants in Boreal Forests on the Eve of the 21 Century”, Glubokoye-Gomel, Belarus, September 11–15, 2000. Gomel, Forest institute NASB, 2000, pp. 193–196.
  15. Pirinen H., Dalman P., Kärenlampi S., Tammisola J., Kokko H. Description of Three New Arctic Bramble Cultivars and Proposal for Cultivar Identification. Agricultural and Food Science in Finland, 1998, vol. 7, no. 4, pp. 455–468. DOI: 10.23986/afsci.5608
  16. Ragnar M., Rytkönen P., Hedh J. Åkerbär. Luleå, Sweden, Black Island Books, 2017. 169 p.
  17. Read P.E., Economou A.S., Hartley C.A., Grout J.M., Fellman C.D. A Summary of Stock Paint Influences on Woody Plant Tissue Culture Success with Special Emphasis on the Ericaceae. Acta Horticulturae, 1988, vol. 227, pp. 476–478. DOI: 10.17660/ActaHortic.1988.227.98
  18. Strik B.C., Yarborough D.E. Blueberry Production Trends in North America, 1992 to 2003, and Predictions for Growth. HortTechnology, 2005, vol. 15, iss. 2, pp. 391–398. DOI: 10.21273/HORTTECH.15.2.0391
  19. Vahejõe K., Albert T., Noormets M., Karp K., Paal T., Starast M., Värnik R. Berry Cultivation in Cutover Peatlands in Estonia: Agricultural and Economical Aspects. Baltic Forestry, 2010, vol. 16, no. 2(31), pp. 264–272.
  20. Yarborough D.E. Cultivated Lowbush Blueberry (Vaccinium angustifolium) Establishment and Management. Journal of Jilin Agricultural University, 2009, vol. 31(5), pp. 556–560.

Obtaining High-Quality Planting Material of Forest Berry Plants by Clonal Micropropagation for Restoration of Cutover Peatlands


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