Use of Plasma-treated Water to Prepare Seeds of Coniferous Species for Sowing. P. 204–211

Complete text of the article:

Download article (pdf, 1.1MB )

UDС

630*23

DOI:

10.37482/0536-1036-2023-5-204-211

Abstract

The article discusses the accelerated cultivation of quality planting material using the method of seed preparation by soaking in water treated in low-temperature plasma discharge. The most promising planting material are seedlings with closed root system. They are grown from improved seed grain in optimal conditions of greenhouses. Among various methods of stimulating germination of the seed germ, it is of interest to influence it with “cold” plasma. With the help of a specially designed device, which allows to treat water with lowtemperature plasma discharge, plasma-treated water for soaking seeds was obtained. The time of water treatment varied from 1 to 4 min. Water activated by plasma had acidity indicators other than those of ordinary water. The time of acidity level restoration was from 2 to 4 days. Germination rates and germination energy were obtained for seeds of Pinus silvestrys and Picea abies soaked in water with different duration of plasma treatment. When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water). Technical germination of pine seeds increased by 11–21 %, for spruce by 3–16 %. The results of research show changes in the structure and composition of the nutrient substance of the seed, in the structure of the germ and the degree of its development. Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %. When seeds were soaked in distilled water, the content of phosphorus in the endosperm decreased by 0.15 %, and the content of sodium – by 0.2 %. For seeds soaked in plasma treated water, phosphorus content decreased by 0.21–0.22 % and sodium content was not observed. Soaking seeds in plasma-treated water stimulates the processes of their preparation for germination and differentiation of germ tissues (formation of seed pods), compared to seeds soaked in water.

Authors

Olga I. Gavrilova*, Doctor of Agriculture, Assoc. Prof.; ResearcherID: AAF-6295-2019, ORCID: https://orcid.org/0000-0002-5618-8239
Kirill V. Gostev, Candidate of Engineering; ResearcherID: HLW-5102-2023, ORCID: https://orcid.org/0000-0003-2286-059X

Affiliation

Petrozavodsk State University, Lenina prosp., 33, Petrozavodsk, 185910, Russian Federation; ogavril@mail.ru, kgostev@petrsu.ru

Keywords

forest nursery, greenhouse, seeds, common pine, common spruce, endosperm, germ, germination, cold plasma spray

For citation

Gavrilova O.I., Gostev K.V. Use of Plasma-treated Water to Prepare Seeds of Coniferous Species for Sowing. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 5, pp. 204–211. (In Russ.). https://doi.org/10.37482/0536-1036-2023-5-204-211

References

1. Gostev K.V., Gavrilova O.I., Gostev V.A. Application of Cold Plasma Spray for Pre-sowing Preparation of Scots Pine Seeds. Vestnik Moskovskogo gosudarstvennogo universiteta lesa – Lesnoy vestnik = Forestry Bulletin, 2014, vol. 18, no. 1, pp. 90–96. (In Russ.).

2. Gostev K.V., Shtykov A.S., Vasiliev A.S. On the Potential of Using Low-temperature Plasma in Industry and Social sphere. Inzhenernyy vestnik Dona = Engineering Bulletin of the Don, 2017. no. 3, 9 p. (In Russ.).

3. Zhigunov A.V. Closed Root Planting Material. Lesnoye khozyaystvo = Forestry, 1995, no. 4, pp. 33–38. (In Russ.)

4. Kasimov A.K. The Development of the Pine Root System in Plantings and Crops Under Mechanical Soil Cultivation. Lesnoy Zhurnal = Russian Forestry Journal, 1995, no. 2–3, pp. 30–38. (In Russ.).

5. Markova I.A., Zhigunov A.V. Specificity of Using Planting Material in Various Subzones of Boreal Forests. Dokl. international conf. Forest, environment and new technologies in Northern Europe. Petrozavodsk, 1993. Ed. Universitet Joensuu, no. 17., 1994, pp. 432–433. (In Russ.).

6. Nakvasina E.N. On the Feeding Rhythms of Annual Spruce Seedlings in a Forest Nursery. Lesnoy Zhurnal = Russian Forestry Journal, 1979, no. 2, pp. 12–16.

7. Novoseltseva A.I., Rodin A.R. Handbook of Forest Nurseries. Moscow, Lesnaya promyshlennost’ Publ., 1983. 280 p. (In Russ.).

8. Gostev K.V., Tikhomirov A.A., Tikhonov E.A. Pulse Generator of Supercooled Plasma. Patent RF, no. RU 131931, 2013. (In Russ.).

9. Yurieva A.L., Gavrilova O.I., Khlyustov V.K. Dynamics of the Formation of the Aboveground Part of Pine Crops During the Early Diagnosis. Petrozavodskiy gosudarstvennyy universitet = Proceedings of the Faculty of Forestry and Engineering, 2008, no. 7, pp. 149–151. (In Russ.). https://doi.org/10.15393/j2.art.2008.1871

10. Akimov R.Yu., Ostroshenko V.V. The Seed Pelleting of Korean Cedar Pine (Pinus koraiensis Siebold et Zucc.) and Amur Larch (Larix amurensis). Agrarnyy vestnik Primor’ya = Agrarian Bulletin of Primorye, vol. 4, pp. 39–41. (In Russ.).

11. Bao D., Luoranen J., Lehto T., Himanen K., Silvennoinen M., Silvennoinen R., Repo T. Biophysical Changes in the Roots of Scots Pine Seedlings During Cold Acclimation and After Frost Damage. Forest Ecology and Management, 2019, vol. 431, pp. 63–72. https://doi.org/10.1016/j.foreco.2018.04.008

12. Goebel D.M., Forrester A.T. Plasma Studies on a Hollow Cathode, Magnetic Multipole Ion Source for Neutral Beam Injection. Rev. Sci. Instrum, 1982, vol. 53, iss. 6, pp. 810–815. https://doi.org/10.1063/1.1137051

13. Goebel D.M., Watkins R.M. High Current, Low Pressure Plasma Cathode Electron Gun. Rev. Sci. Instrum, 2000, vol. 71, iss. 2, pp. 388–398. https://doi.org/10.1063/1.1150212

14. Gushenets V.I., Oks E.M., Yushkov G.Yu., Rempe N.G. Current Status of the Plasma Emission Electronics. I. Basic Physical Processes. Laser and Particle Beams, 2003, vol. 21, iss. 2, pp. 123–138. https://doi.org/10.1017/S0263034603212027

15. Himanen K., Helenius P., Ylioja T., Nygren M. Intracone Variation Explains Most of the Variance in Picea Abies Seed Weight. Implications for Seed Sorting. Canadian Journal of Forest Research, 2016, vol. 46, no. 4, pp. 470–477. https://doi.org/10.1139/cjfr-2015-0379

16. Himanen K. Seed Quality Attributes in Seedling Production of Norway Spruce (Picea abies (L.) Karst.). Dissertationes Forestales, 2018, vol. 261, 74 p. https://doi.org/10.14214/df.261

17. Lilja A., Himanen K. Other Seed and Cone Diseases. The American Phytopathology Society, 2018.

18. Miransari М., Smith D.L. Plant Hormones and Seed Germination. Environmental and Experimental Botany, 2014, vol. 99, pp. 110–121. https://doi.org/10.1016/j.envexpbot.2013.11.005