The Use of Lighting of Various Spectral Ranges for Clonal Micropropagation of Forest Berry Plants. P. 82–93

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634.7

DOI:

10.37482/0536-1036-2022-6-82-93

Abstract

Creation of plantations of the most popular species of forest berry plants with high nutritional and medicinal value is advisable in connection with the reduction of such natural resources and the increased demand for berry products, as well as for the biological reclamation of depleted peat deposits. The paper shows the research results of clonal micropropagation of forest berry plants (cranberry, American cranberry, half-high blueberry, Arctic bramble, lingonberry, Kamchatka bilberry) of promising cultivars and forms using various types of lighting (white LED lamps, LED lamps with a combination of white, red and blue spectra, as well as white fluorescent lamps). Plants were cultivated using WPM and MS nutrient media with the addition of cytokinins 2-iP and 6-BAP in various concentrations. The largest number (3.3–16.9 pcs) and the maximum total length (13.8–251.1 cm) of microshoots of cranberry (Dar Kostromy cultivar, hybrid form 1-15-635), American cranberry (Ben Lear cultivar, hybrid form 1-23-3), Arctic bramble (Anna cultivar, hybrid form K-1), lingonberry (Kostromskaya rozovaya and Rubin cultivars) and Kamchatka bilberry (Sakhalinskaya and Kurilskaya forms) were observed under lighting by LED lamps with a combination of white, red and blue spectra at the “proper micropropagation” stage. The formation of the largest number (13.1 pcs) and the maximum length (98.7 cm) of microshoots of half-high blueberry (Northblue cultivar, hybrid form 23-1-11) was observed under lighting by white fluorescent lamps. There were no significant differences in biometric parameters of plants under different types of lighting depending on cultivars and forms. The use of LED lamps with a combination of white, red and blue spectra has a significant effect on the formation of microshoots of forest berry plants during clonal micropropagation.

Authors

Sergey S. Makarov^1,2*, Doctor of Agriculture, Senior Research Scientist; ResearcherID: AAK-9829-2021, ORCID: https://orcid.org/0000-0003-0564-8888
Mikhail T. Upadyshev³, Doctor of Agriculture, Corresp. Member of RAS, Chief Research Scientist; ResearcherID: AAE-1086-2022, ORCID: https://orcid.org/0000-0003-1069-3771
Irina B. Kuznetsova⁴, Candidate of Agriculture, Assoc. Prof.; ResearcherID:AAB-4568-2021, ORCID: https://orcid.org/0000-0001-5011-3271
Aleksandra V. Zaushintsena⁵, Doctor of Biology, Prof.; ORCID: https://orcid.org/0000-0003-4645-828X
Elena I. Kulikova⁶, Candidate of Agriculture, Head of Department; ResearcherID: AAL-8290-2021, ORCID: https://orcid.org/0000-0002-5981-2690
Elena A. Surina⁷, Candidate of Agriculture; ResearcherID: AAD-6192-2019, ORCID: https://orcid.org/0000-0002-8159-8977

Affiliation

¹Central European Forest Experimental Station, prosp. Mira, 134, Kostroma, 156013, Russian Federation; makarov_serg44@mail.ru*
²Northern (Arctic) Federal University named after M.V. Lomonosov, Naberezhnaya Severnoy Dviny, 17, Arkhangelsk, 163002, Russian Federation; makarov_serg44@mail.ru
³Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery, ul. Zagor’yevskaya, 4, Moscow, 115598, Russian Federation; vstisp@vstisp.org
⁴Kostroma State Agricultural Academy, Uchebnyy gorodok, Karavaevskaya s/a, 34, p. Karavaevo, Kostroma District, Kostroma Region, 156530, Russian Federation; sonnereiser@yandex.ru
⁵Kemerovo State University, ul. Krasnaya, 6, Kemerovo, 650000, Russian Federation; alexaz58@yandex.ru
⁶Vologda State Dairy Farming Academy named after N.V. Vereshchagin, ul. Shmidta, 2, s. Molochnoe, Vologda, Vologda Region, 160555, Russian Federation; elena-kulikova@list.ru
⁷Northern Research Institute of Forestry, ul. Nikitova, 13, Arkhangelsk, 163062, Russian Federation; surina_ea@sevniilh-arh.ru

Keywords

clonal micropropagation, in vitro, lighting, lighting effects on plants, LED lamps, berry plants, cranberry, blueberry, Arctic bramble, lingonberry, Kamchatka bilberry

For citation

Makarov S.S., Upadyshev M.T., Kuznetsova I.B., Zaushintsena A.V., Kulikova E.I., Surina E.A. The Use of Lighting of Various Spectral Ranges for Clonal Micropropagation of Forest Berry Plants. Lesnoy Zhurnal = Russian Forestry Journal, 2022, no. 6, pp. 82–93.
(In Russ.). https://doi.org/10.37482/0536-1036-2022-6-82-93

References

1. Byadovsky I.A. The Effect of Led Light Sources with Varied Spectral Composition on the in vitro Rooting Bility of Garden Strawberry (Fragaria×ananassa). Trudy po prikladnoj botanike, genetike i selekcii = Proceedings on applied botany, genetics and breeding, 2019, vol. 180, no. 1, pp. 33–37. (In Russ.). https://dx.doi.org/10.30901/2227-8834-2019-1-33-37

2. Byadovsky I.A., Upadyshev M.T. Clonal Micropropagation of Fruit Crops. Moscow, FSBSO ARHCBAN Publ., 2020. 69 p. (In Russ.).

3. Gud’ L.A., Kalashnikova E.A., Tarakanov I.G. Influence of Light of Different Spectral Range on the Morphogenesis of Blackberry and Raspberry in vitro. Forestry information, 2019, no. 2, pp. 97–102. (In Russ.). https://doi.org/10.24419/LHI.2304-3083.2019.2.09

4. Kalashnikova E.A. Plant Cellular Engineering. Moscow, RSAU – MTAA Publ., 2012. 317 p. (In Russ.).

5. Korenev I.A., Tyak G.V., Makarov S.S. Creation of New Cultivars of Forest Berry Plants and the Prospects of Their Intensive Reproduction (in vitro). Forestry information, 2019, no. 3, pp. 180–189. (In Russ.). https://doi.org/10.24419/LHI.2304-3083.2019.3.15

6. Makarov S.S., Kuznetsova I.B., Smirnov V.S. Improving Technology of Clonal Micropropagation of Arctic Bramble (Rubus arcticus L.). Forestry information, 2018, no. 4, pp. 91–97. (In Russ.). https://doi.org/10.24419/LHI.2304-3083.2018.4.09

7. Makarov S.S., Kuznetsova I.B., Upadyshev M.T., Rodin S.A., Chudetsky A.I. Clonal Micropropagation of Cranberry (Oxycoccus palustris Pers.). Food Processing: Techniques and Technology, 2021, vol. 51, iss. 1, pp. 67–76. (In Russ.). https://doi.org/10.21603/2074-9414-2021-1-67-76

8. Agricultural Biotechnology and Bioengineering. Ed. by V.S. Shevelukha. Moscow, URSS Publ., 2015. 710 p. (In Russ.).

9. The Strategy for the Development of the Forest Complex of the Russian Federation for the Period up to 2030: Approved by the Order of the Government of the Russian Federation Dated February 11, 2021 No. 312-р. Режим доступа: http://static.government.ru/media/files/pFdqtWFH8y9SfQjDE0Xnwd8eXWoJJMYB.pdf (дата обращения: 20.10.22). (In Russ.).

10. Tikhomirov A.A., Ushakova S.A. Scientific and Technological Foundations for the Formation of a Phototrophic Link in Biological and Technical Life Support Systems. Krasnoyarsk, SibSAU Publ., 2016. 200 p. (In Russ.).

11. Tyak G.V., Kurlovich L.E., Tyak A.V. Biological Recultivation of Degraded Peatlands by Creating Forest Berry Plants. Vestnik of Kazan State Agricultural University, 2016, vol. 11, no. 2(40), pp. 43–46. (In Russ.). https://doi.org/10.12737/20633

12. Upadyshev M.T. Spectrum Composition of Light at Microinultiplication of Plants in Species Rubus and Sorbus. Doklady Rossiyskoy akademii sel’skokhozyaystvennykh nauk, 2002, no. 6, pp. 16–19. (In Russ.).

13. Upadyshev M.T. Effect of Light of Different Spectral Composition during Micropropagation of Fruit and Berry Crops. Aktual’naya biotekhnologiya, 2018, no. 3(26), p. 521. (In Russ.).

14. Anderson W.C. Propagation of Rhododendrons by Tissue Culture. 1. Development of a Culture Medium for Multiplication of Shoots. Proceedings of the International Plant Propagator’s Society, 1975, vol. 25, pp. 129–135.

15. Bussières J., Rochefort L., Lapointe L. Cloudberry Cultivation in Cutover Peatland: Improved Growth on Less Decomposed Peat. Canadian Journal of Plant Science, 2015, vol. 95, no. 3, pp. 479–489. https://doi.org/10.4141/cjps-2014-299

16. Cope K., Bugbee B. Spectral Effects of Three Types of White Light-Emitting Diodes on Plant Growth and Development: Absolute versus Relative Amounts of Blue Light. HortScience, 2013, vol. 48, iss. 4, pp. 504–509. https://doi.org/10.21273/HORTSCI.48.4.504

17. Hung C.D., Hong C.-H., Kim S.-K., Lee K.-H., Park J.-Y., Nam M.-W., Choi D.-H., Lee H.-I. LED Light for in vitro and ex vitro Efficient Growth of Economically Important Highbush Blueberry (Vaccinium corymbosum L.). Acta Physiologiae Plantarum, 2016, vol. 38, art. 152. https://dx.doi.org/10.1007/s11738-016-2164-0

18. Kang J.H., Kumar S.K., Atulba S.L.S., Jeong B.R., Hwang S.J. Light Intensity and Photoperiod Influence the Growth and Development of Hydroponically Grown Leaf Lettuce in a Closed-Type Plant Factory System. Horticulture, Environment, and Biotechnology, 2013, vol. 54, iss. 6, pp. 501–509. https://doi.org/10.1007/s13580-013-0109-8

19. Lloyd G.B., McCown В.Н. Commercially-Feasible Micropropagation of Mountain Laurel, Kalmia latifolia, by Use of Shoot-Tip Culture. Combined Proceeding. International Plant Propagatiors’ Society, 1980, vol. 30, pp. 421–427.

20. Makarov S.S., Kuznetsova I.B., Chudetsky A.I., Rodin S.A. Obtaining High-Quality Planting Material of Forest Berry Plants by Clonal Micropropagation for Restoration of Cutover Peatlands. Lesnoy Zhurnal = Russian Forestry Journal, 2021, no. 2, pp. 21–29. https://doi.org/10.37482/0536-1036-2021-2-21-29

21. Murashige T., Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Phisiologia Plantarum, 1962, vol. 15, iss. 3, pp. 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

22. Noormets M., Karp K., Paal T. Recultivation of Opencast Peat Pits with Vaccinium Culture in Estonia. WIT Transactions on Ecology and the Environment. Vol. 64: Ecosystems and Sustainable Development IV, 2003, vol. 2, pp. 1005–1014. https://doi.org/10.2495/ECO030242

23. Palozzi J.E. Peatland Plant-Soil Feedbacks Dictate Ecosystem Properties and Processes. Electronic Thesis and Dissertation Repository. Canada, 2017. 86 p. Available at: https://ir.lib.uwo.ca/etd/4511 (accessed 25.03.21).

24. Production of Berries in Peatlands. Peatland Ecology Research Group. Guide Produced under the Supervision of Line Rochefort and Line Lapointe. Quebec, Université Laval, 2009. 134 p.

25. Vahejõe K., Albert T., Noormets M., Karp K., Paal T., Starast M., Värnik R. Berry Cultivation in Cutover Peatlands in Estonia: Agricultural and Economical Aspects. Baltic Forestry, 2010, vol. 16, no. 2(31), pp. 264–272.