The Content of Phenolic Compounds in Lichens in the Tectonic Fault Zones. P. 198–213

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UDС

582.29:550.47

DOI:

10.37482/0536-1036-2022-4-198-213

Abstract

The paper studies the influence of geoecological factors on the content of phenolic compounds (PCs) in lichens of the genus Cladonia in the Velsko-Ustyansky and Kholmogorsky tectonic knots (TKs). Lichens were sampled from the test plots (TPs) laid out in the center of the knots and outside the knots (control). The geochemical situation, soil-climatic and environmental conditions in the zone of TKs influence the lichens chemical composition. It is found that the ash content of lichens growing in the center of the TK is 1.8–2.3 times higher than in the control, which indicates a significant accumulation of lithogenic elements in the lichen thallus in the tectonic fault zone. Lichens are exposed to multifactorial adverse effects (increased radiation background and heat flux, electromagnetic radiation, electric and magnetic fields, etc.) in the territory of TKs. This provokes excessive generation of active oxygen radicals and contributes to the rearrangement of lichen metabolism, changing the dynamics of accumulation of PCs and their biosynthesis. The content of the water-insoluble fraction of PCs in lichens was found to increase from the control point to the center of the TK, while the relative share of the water-soluble fraction of PCs in the total composition decreases. This is probably due to an increase in oxidative processes and a decrease in reductive processes in lichens under adverse conditions. The paper shows that the biosynthesis of PCs in lichens is most active in the spring-summer (April and July) vegetation period. The impact of geoecological factors enhances the biosynthesis of secondary metabolites of phenolic nature in lichens in the conditions of the TKs: usnic acid (UA) and atranorin. The content of UA in the composition of the water-insoluble fraction of PCs in lichens growing in the center of the TK is 1.5–1.7 times higher during the spring-summer vegetation period than in lichens growing outside the TK. The quantitative distribution of the water-insoluble fraction of PCs depends on the lichen thallus part and has a gradient character. Their content in the upper growing parts of lichens growing in the center of the TK and in the control is 2 and 1.7 times higher than in the old zones, respectively. Thus, PCs in lichens are active metabolites and their content in thallus can serve as a biomarker of the state of the environment.

Authors

Irina A. Palamarchuk, Candidate of Chemistry, Senior Research Scientist; ResearcherID: AAF-5454-2019, ORCID: https://orcid.org/0000-0002-2947-1370
Marina E. Belousova*, Junior Research Scientist; ResearcherID: AAL-7068-2020, ORCID: https://orcid.org/0000-0002-6099-1358
Olga S. Brovko, Candidate of Chemistry, Leading Research Scientist, Assoc. Prof.; ResearcherID: AAF-5387-2019, ORCID: https://orcid.org/0000-0002-1961-7831
Vadim V. Staritsyn, Candidate of Agriculture, Research Scientist;
ResearcherID: J-3588-2018, ORCID: https://orcid.org/0000-0001-6891-1247
Artem D. Ivakhnov, Candidate of Chemistry, Senior Research Scientist; ResearcherID: U-4822-2019, ORCID: https://orcid.org/0000-0003-2822-9192
Anatoliy A. Sloboda, Junior Research Scientist; ResearcherID: AAL-3955-2021, ORCID: https://orcid.org/0000-0002-1415-9050

Affiliation

N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences, Naberezhnaya Severnoy Dviny, 23, Arkhangelsk, 163002, Russian Federation; irpalamarchuk@mail.ru, marinabelousovae@yandex.ru*, brovko-olga@rambler.ru, Corwin87@mail.ru, ivahnov-tema@yandex.ru, sloboda.iepn@yandex.ru

Keywords

lichens, Cladonia, tectonic knot, phenolic compounds, lichen acids, waterinsoluble phenolic compounds, water-soluble phenolic compounds, usnic acid, atranorin, Velsko-Ustyansky tectonic knot, Kholmogorsky tectonic knot

For citation

Palamarchuk I.A., Belousova M.E., Brovko O.S., Staritsyn V.V., Ivakhnov A.D., Sloboda A.A. The Content of Phenolic Compounds in Lichens in the Tectonic Fault Zones. Lesnoy Zhurnal = Russian Forestry Journal, 2022, no. 4, pp. 198–213. (In Russ.). https://doi.org/10.37482/0536-1036-2022-4-198-213

References

1. Abdrakhimova Y.R., Valiyeva A.I. Secondary Plant Metabolites: Physiological and Biochemical Aspects. Part 3. Phenolic Compounds. Kazan, Kazan University Publ., 2012. 40 p. (In Russ.).

2. Belyaev V.V., Neverov N.A., Staritsyn V.V., Boytsova T.A. Properties of Some Components of Phytocenoses Growing on the Territory of Tectonic Knots (Arkhangelsk Region). The Bulletin of KrasGAU, 2018, no. 4, pp. 197–204. (In Russ.).

3. Boyarskikh I.G., Kulikova A.I. Variability of Cytogenetic Disturbances in Lonicera caerulea (Blue Honeysuckle) Population in аn Active Fault Zone. Ecological Genetics, 2017, vol. 15, no. 2, pp. 62–70. (In Russ.). https://doi.org/10.17816/ecogen15262-70

4. Brovko O.S., Palamarchuk I.A., Sloboda A.A., Boytsova T.A., Gagushkina A.A., Valchuk N.A. The Influence of Stress Factors on the Chemical Composition of Lichens Genus Сladonia of Euro-Arctic Region. Advances in Current Natural Sciences, 2016, no. 8, pp. 20–24. (In Russ.). https://doi.org/10.17513/use.36072

5. Weinstein E.A. Lichen Symbiosis and Physiological and Biochemical Regulation of the Relationship between Fungal and Algal Components: Dr. Biol. Sci. Diss. Leningrad, 1987. 234 p. (In Russ.).

6. Weinstein E.A., Takhtadzhyan E.A. Physiological Changes in the Lichen Alga Trebuxia during Cultivation. Fiziologiya rastenij = Soviet Plant Physiology, 1981, vol. 28, no. 5, pp. 1037–1044. (In Russ.).

7. Voskresenskaya O.L., Alyabysheva E.A., Polovnikova M.G. Great Workshop on Bioecology. Part 1. Yoshkar-Ola, MarSU Publ., 2006. 107 p. (In Russ.).

8. Zagoskina N.V., Nikolaeva T.N., Lapshin P.V., Zavarzin A.A., Zavarzina A.G. Water-Soluble Phenolic Compounds in Lichens. Mikrobiologiya = Microbiology, 2013, vol. 82, no. 4, pp. 434–441. (In Russ.). https://doi.org/10.7868/S0026365613030166

9. Zagoskina N.V., Nikolaeva T.N., Lapshin P.V., Zavarzina A.G., Zavarzin A.A. On the Content of Phenolic Compounds in Various Lichen Species of the Kola Peninsula. Khimija Rastitel’nogo Syr’ja = Chemistry of plant raw material, 2011, no. 4, pp. 245–249. (In Russ.).

10. Kaplin V.G. Bioindication of the Ecosystems State. Samara, Samarskaya GSKhA Publ., 2001. 143 p. (In Russ.).

11. Kutinov Yu.G., Chistova Z.B. Hierarchical Series of Manifestations of Alkaline-Ultrabasic Magmatism of the Arkhangelsk Diamond-Bearing Province. Their Representation in Geological and Geophysical Data. Arkhangelsk, Pravda Severa Publ., 2004. 233 p.(In Russ.).

12. Kutinov Yu.G., Chistova Z.B., Belyaev V.V., Burlakov P.S. The Northern Part of the Russian Plate: Effects of the Tectonic Structures on the Environment (Case Study for Arkhangelsk Region). Vestnik Kamchatskoy regional’noy assotsiatsii “Uchebno-nauchnyy tsentr”. Seriya: Nauki o Zemle = Bulletin of Kamchatka Regional Association “Educational-Scientific Center”. Earth Sciences, 2009, vol. 2, no. 14, pp. 77–89. (In Russ.).

13. Lithosphere and Hydrosphere of the European North of Russia. Geoecological Problems. Ed. by F.N. Yudahin. Yekaterinburg, UB RAS Publ., 2001. 407 p. (In Russ.).

14. Lyskova N.S., Bazarnova Iu.G., Kruchina-Bogdanov I.V. Study of the Composition and Properties of Secondary Metabolites of Lichen Usnea barbata. Khimija Rastitel’nogo Syr’ja = Chemistry of plant raw material, 2018, no. 1, pp. 121–127. (In Russ.). https://doi.org/10.14258/jcprm.2018011966

15. Malov A.I., Kutinov Y.G., Chistova Z.B., Beljaev V.V., Gofarov M.Y., Polyakova E.V., Staritsyn V.V., Durynin S.N., Mineev A.L., Antonovskaya G.N., Kapustian N.K., Vaganova N.V., Morozov A.N., Konechnaya Ya.V. Fundamentals of Environmentally-Sound Mastering Technologies for Natural Resources of the Western Arctic Sector of the Russian Federation. Part 2. Georesources, Geoenergetics, Geopolitics, 2014, no. 1(9). (In Russ.).

16. Meychik N.R., Lyubimova E.G. Ion-Exchange Properties of the Cell Wall of the Reindeer Lichen Cladonia rangiferina. Fiziologiya rastenij = Russian Journal of Plant Physiology, 2010, vol. 57, no. 2, pp. 273–279. (In Russ.).

17. Moiseeva E.N. On the Localization of Enzymes and Lichen Acids in Thallus of Lichens. Botanicheskii Zhurnal, 1959, vol. 44, no. 8, pp. 1128–1134. (In Russ.).

18. Palamarchuk I.A., Brovko O.S., Belyaev V.V., Bogolitsyn K.G., Boitsova T.A., Zhiltsov D.V., Sloboda A.A., Valchuk N.A. Influence of the Environmental Geoecological Factors on the Biochemical Indicators of Lichens in the Territory of the Velsk-Ustyansk Tectonic Node (Arkhangelsk Region). Khimija Rastitel’nogo Syr’ja = Chemistry of plant raw material, 2018, no. 4, pp. 215–224. (In Russ.). https://doi.org/10.14258/jcprm.2018043803

19. Prusakova L.D., Kefeli V.I., Belopukhov S.L., Vakulenko V.V., Kuznetsova S.A. Role of Phenolic Compounds in Plants. Agrohimia = Eurasian Soil Science, 2008, no. 7, pp. 86–96. (In Russ.).

20. Ravinskaya A.P., Weinstein E.A. Influence of Some Environmental Factors on the Content of Lichen Substances. Ekologia = Russian Journal of Ecology, 1975, no. 3, pp. 82–85. (In Russ.).

21. Ravinskaja A.P., Vainschtein E.A. Chemotaxonomic Significance of Changes in the Content of Lichen Acids. Novosti sistematiki nizshikh rastenii, 1975, vol. 12, pp. 266–273. (In Russ.).

22. Sokolov D.N., Luzina O.A., Salahutdinov N.F. Usnic Acid: Preparation, Structure, Properties and Chemical Transformations. Uspekhi Khimii = Russian Chemical Reviews, 2012, vol. 81, no. 8, pp. 747–768. (In Russ.).

23. Tolpysheva T.Yu., Shishkonakova E.A. Lichens of the Natural Park “Numto”. A Short Determinant. Yekaterinburg, Assorti Publ., 2018. 187 p. (In Russ.).

24. Shevchenko V.P., Starodymova D.P., Kutenkov S.A., Vinogradova A.A., Gordeev V.V., Demina L.L., Ivanova Yu.A., Filippov A.S. Contents of Heavy Metals in Fruticose Epiphytic Lichens of Karelia as Indicator of Atmospheric Transport of Pollutants. Sovremennye Problemy Nauki i Obrazovaniya = Modern problems of science and education, 2011, no. 3. (In Russ.).

25. Shcherbakova A.I. Lichens with High Antioxidant Activity. Vestnik of Volga State University of Technology Series “Forest: Ecology. Nature Management”, 2018, no. 4(40), pp. 75–84. (In Russ.).

26. Ahmadjian V. Studies on the Isolation and Synthesis of Bionts of the Cyanolichen Peltigera canina (Peltigeraceae). Plant Systematics and Evolution, 1989, vol. 165, pp. 29–38. https://doi.org/10.1007/BF00936032

27. Ahmadjian V., Paracer P. Symbiosis: An Introduction in Biological Association. LA, Clark University Press, 1986. 212 р.

28. Ahti T., Stenroos S., Moberg R. Nordic Lichen Flora. Vol. 5. Cladoniaceae. Uppsala, Museum of Evolution, 2013. 117 p.

29. Aoussar N., Rhallabi N., Mhand R.A, Manzali R., Bouksaim M., Douira A., Mellouki F. Seasonal Variation of Antioxidant Activity and Phenolic Content of Pseudevernia furfuracea, Evernia prunastri and Ramalina farinacea from Morocco. Journal of the Saudi Society of Agricultural Sciences, 2020, vol. 19, iss. 1, pp. 1–6. https://doi.org/10.1016/j.jssas.2018.03.004

30. Belyaev V., Bogolitsyn K., Brovko O., Kutinov Yu., Neverov N., Palamarchuk I., Boytsova T., Chukhchin D., Zhiltsov D., Gorshkova N. Influence of Tectonic Faults on the Conditions and Properties of Some Components of a Biogeocenosis in a Subarctic Area. European Journal of Environmental Sciences, 2019, vol. 9, no. 1, pp. 5–11. https://doi.org/10.14712/23361964.2019.1

31. Brovko O.S., Ivakhnov A.D., Palamarchuk I.A., Boitsova T.A. Supercritical Fluid Extraction of Usnic Acid from Lichen of Cladonia Genus. Russian Journal of Physical Chemistry B, 2017, vol. 11, iss. 8, pp. 1306–1311. https://doi.org/10.1134/S1990793117080024

32. Kosanić M., Ranković B., Stanojković T., Rančić A., Manojlović N. Cladonia Lichens and Their Major Metabolites as Possible Natural Antioxidant, Antimicrobial and Anticancer Agents. LWT – Food Science and Technology, 2014, vol. 59, iss. 1, pp. 518–525. https://doi.org/10.1016/j.lwt.2014.04.047

33. Manojlovic N.T., Solujic S., Sukdolak S. Antimicrobial Activity of an Extract and Antraquinones from Caloplaca shaeveri. The Lichenologist, 2002, vol. 34, iss. 1, pp. 83–85. https://doi.org/10.1006/lich.2001.0365

34. Molnár K., Farkas E. Current Results on Biological Activities of Lichen Secondary Metabolites: A Review. Zeitschrift für Naturforschung C, 2010, vol. 65, iss. 3-4, pp. 157–173. https://doi.org/10.1515/znc-2010-3-401

35. Purvis O.W., Williamson B.J., Spiro B., Udachin V., Mikhailova I.N., Dolgopolova A. Lichen Monitoring as a Potential Tool in Environmental Forensics: Case Study of the Cu Smelter and Former Mining Town of Karabash, Russia. Geological Society, London, Special Publication, 2013, vol. 384, no. 1, pp. 133–146. https://doi.org/10.1144/SP384.6

36. Rola K., Latkowska E., Myśliwa-Kurdziel B., Osyczka P. Heavy-Metal Tolerance of Photobiont in Pioneer Lichens Inhabiting Heavily Polluted Sites. Science of the Total Environment, 2019, vol. 679, pp. 260–269. https://doi.org/10.1016/j.scitotenv.2019.05.002

37. Rola K., Osyczka P. Temporal Changes in Accumulation of Trace Metals in Vegetative and Generative Parts of Xanthoria parietina Lichen Thalli and Their Implications for Biomonitoring Studies. Ecological Indicators, 2019, vol. 96, part 1, pp. 293–302. https://doi.org/10.1016/j.ecolind.2018.09.004

38. Smeds A.I., Kytöviita M.-M. Determination of Usnic and Perlatolic Acids and Identification of Olivetoric Acids in Northern Reindeer Lichen (Cladonia stellaris) Extracts. The Lichenologist, 2010, vol. 42 (6), pp. 739–749. https://doi.org/10.1017/S002428291000037X

39. Swain T., Hillis W.E. The Phenolic Constituents of Prunus domestica I. – The Quantitative Analysis of Phenolic Constituents. Journal of the Science of Food and Agriculture, 1959, vol. 10, iss. 1, pp. 63–68. https://doi.org/10.1002/jsfa.2740100110

40. Taguchi H., Sankawa U., Shibata S. Biosynthesis of Natural Products. VII. Biosynthesis of Usnic Acid in Lichens. Seasonal Variation Observed in Usnic Acid Biosynthesis. Chemical and Pharmaceutical Bulletin, 1969, vol. 17, iss. 10, pp. 2061–2064. https://doi.org/10.1248/cpb.17.2061