Features of Adaptation of Different Forms of Scots Pine under Conditions of Prolonged Excessive Soil Moistening

Complete text of the article:

Download article (pdf, 0.6MB )

UDС

581.5:58.087

DOI:

https://doi.org/10.37482/0536-1036-2021-2-30-44

Abstract

Intrapopulation variability of biochemical traits and cambial growth shows the adaptive ability of pine trees in different growth conditions. There is a genetically determined process of activation of defense systems in pine trees under unfavorable environmental conditions. Structural and functional rearrangement of the assimilating apparatus due to seasonal development ensures increased tree resilience and the passage of ontogenesis under the influence of stress factors within the reaction norm. This work aims at the study of adaptive ability of Pinus sylvestris L., which differs in the apophysis form of seed scales under conditions of prolonged excessive soil moistening in northern taiga. Studies were carried out in shrubby-sphagnum pine forests on boggy upland peat soils in the mouth of the Northern Dvina River. Trees with primary apophysis variations (forms) of seed scales were identified on the sample plots. Samples of 1, 2 and 3-year-old needles were taken in order to determine biochemical traits in 10 trees of each pine form isolated by type of apophysis, in different calendar periods (from May to December, 2016). The content of free proline, water-soluble proteins, and ascorbic acid was determined in the needles by the spectrophotometric method. Wood cores were sampled from 52 trees at a height of 1.3 m and width of the annual layer was determined in each selected form. Relative dendrochronological parameters were calculated along with the absolute value of radial growth. Meteorological parameters at the study sites (air temperature and precipitation) were determined from data of the Arkhangelsk Weather Station. The studies show the similarities and differences of trees of various forms in the seasonal dynamics of stress metabolites content in needles of different ages in relation to meteorological factors and phenological phases of pine vegetative organs in the Northern Dvina mouth. Trees with flat form of apophysis inherent more intensive accumulation of ascorbic acid and proline in 2-year-old needles in late October. This indicates increased activation of their protective reactions before overwintering compared to trees with convex form of apophysis. Patterns of variability in cambial growth of trees of different forms in time series were found. It was observed that trees with flat form of apophysis are more strongly affected by maladaptive (stressful) environmental factors.

Authors

Sergei N. Tarkhanov, Doctor of Biology; Senior Research Scientist; ResearcherID: ABG-7237-2020,
ORCID: https://orcid.org/0000-0001-9037-8995
Ekaterina A. Pinaevskaya, Candidate of Biology, Research Scientist; ResearcherID: ABB-6293-2020,
ORCID: https://orcid.org/0000-0003-1877-1412
Yuliya E. Aganina, Postgraduate Student; ResearcherID: ABB-6305-2020,
ORCID: https://orcid.org/0000-0002-6069-8979

Affiliation

N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences, Naberezhnaya Severnoy Dviny, 23, Arkhangelsk, 163000, Russian Federation; е-mail: tarkse@yandex.ru, aviatorov8@mail.ru, julja-a30@rambler.ru

Keywords

Scots pine, apophysis form of seed scales, biochemical parameters, radial growth, adaptation, prolonged excessive soil moistening

For citation

Tarkhanov S.N., Pinaevskaya E.A., Aganina Yu.E. Features of Adaptation of Different Forms of Scots Pine under Conditions of Prolonged Excessive Soil Moistening. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 2, pp. 30–44. DOI: 10.37482/0536-1036-2021-2-30-44

References

1. Abaturova M.P. Study of Primary Morphological Traits of Norway Spruce. Research Basis of Selection of Coniferous Woody Species. Moscow, Nauka Publ., 1978, pp. 87–98.
2. Aref’ev S.P. Assessment of the Stability of the Siberian Stone Pine Forests in the Western Siberian Plain. Ekologiya [Russian Journal of Ecology], 1997, no. 3, pp. 175–183.
3. Bitvinskas T.T. Dendroclimatic Studies. Leningrad, Gidrometeoizdat Publ., 1974. 172 p.
4. Large Workshop “Biochemistry”. Laboratory Classes. Content by M.G. Kusakina, V.I. Suvorov, A.A. Chudinova. Perm, PSU Publ., 2012. 148 p.
5. Bukharina I.L., Kuz’min P.A., Sharifullina A.M. Organic Low Molecular Weight Compounds Contents of Tree Leaves under Technogenic Press. Lesovedenie [Russian Journal of Forest Science], 2014, no. 2, pp. 20–26.
6. Vaganov E.A., Terskov I.A. Analysis of Tree Growth by the Structure of Tree Rings. Novosibirsk, Nauka Publ., 1977. 93 p.
7. Vaganov E.A., Shiyatov S.G., Mazepa V.S. Dendroclimatic Studies in Ural-Siberian Subarctic. Novosibirsk, Nauka Publ., 1996. 246 p.
8. Vidyakin A.I. Phenes of Woody Plants: Identification, Scaling and Use in Population Studies (an Example of Pinus sylvestris L.). Ekologiya [Russian Journal of Ecology], 2001, no. 3, pp. 197–202. DOI: 10.1023/A:1011310111062
9. Voskresenskaya O.L., Alyabysheva E.A., Polovnikova M.G. Large Workshop on Bioecology. Part 1. Yoshkar-Ola, MarSU Publ., 2006. 108 p.
10. State Standard. GOST 16128–70. Forest Management Trial Areas. Methods of Laying out. Moscow, Izdatel’stvo standartov, 1971. 23 p.
11. Kalugina O.V., Mikhailova T.A., Shergina O.V. Biochemical Adaptation of Scots Pine (Pinus sylvestris L.) to Technogenic Pollution. Sibirskiy Ekologicheskiy Zhurnal [Contemporary Problems of Ecology], 2018, vol. 25, no. 1, pp. 98–110. DOI: 10.15372/SEJ20180109
12. Mamayev S.A. Types of Intraspecific Variability of Woody Plants (on the Example of the Pinaceae Family in the Urals). Moscow, Nauka Publ., 1972. 284 p.
13. Molotkov P.I., Patlay I.N., Davydova N.I. et al. Breeding of Forest Species. Moscow, Lesnaya promyshlennost’ Publ., 1982. 224 p.
14. Pravdin L.F. Scots Pine. Variability, Intraspecific Systematics and Breeding. Moscow, Nauka Publ., 1964. 192 p.
15. Sudachkova N.E. Metabolism of Conifers and Wood Formation. Novosibirsk, Nauka Publ., 1977. 222 p.
16. Sudachkova N.E., Milyutina I.L., Romanova L.I. Biochemical Adaptation of Conifers to the Stressful Conditions of Siberia. Novosibirsk, Geo Publ., 2012. 178 p.
17. Tabalenkova G.N., Malyshev R.V., Kuzivanova O.A., Atojan M.S. Seasonal Changes in Soluble Protein and Free Amino Acid Content in Buds of Some Woody Plants. Rastitelnye Resursy, 2019, vol. 55, no. 1, pp. 113–121. DOI: 10.1134/S0033994619010126
18. Tarkhanov S.N., Aganina Yu.E., Pakhov A.S. Seasonal Variability of Biochemical Characteristics and a Defect in the Needless of Different Forms of Pinus sylvestris under Stress Conditions in the Northern Taiga. Lesnoy Vestnik [Forestry Bulletin], 2018, vol. 22, no. 1, pp. 5–12. DOI: 10.18698/2542-1468-2018-1-5-12
19. Tarkhanov S.N., Pinaevskaya E.A., Aganina Y.E. Adaptive Responses of Morphological Forms of Pine (Pinus sylvestris L.) under Stressful Conditions of the Northern Taiga (in the Northern Dvina Basin). Sibirskiy Ekologicheskiy Zhurnal [Contemporary Problems of Ecology], 2018, vol. 25, no. 4, pp. 425–437. DOI: 10.15372/SEJ20180404
20. Tarkhanov S.N., Pinaevskay E.A., Anshukova Yu.E. Morpho-Structural Features and Variability of Biochemical Characters of Pinus sylvestris (Pineceae) Variants under Overwetting of Soils in the Northern Taiga. Rastitelnye Resursy, 2014, vol. 50, no. 4, pp. 567–578.
21. Chupakhina G.N., Maslennikov P.V. Plant Adaptation to Oil Stress. Ekologiya [Russian Journal of Ecology], 2004, no. 5, pp. 330–335. DOI: 10.1023/B:RUSE.0000040681.75339.59
22. Shiyatov S.G. Dendrochronology of the Upper Forest Boundary in the Ural. Moscow, Nauka Publ., 1986. 137 p.
23. Shiyatov S.G., Vaganov E.A., Kirdyanov A.V., Kruglov V.B., Mazepa V.S., Naurzbayev M.M., Khantemirov R.M. Methods of Dendrochronology. Part 1. The Basics of Dendrochronology. Collection and Obtaining of Tree-Ring Information. Krasnoyarsk, KSU Publ., 2000. 80 p.
24. Shchekalev R.V., Tarkhanov S.N. The Radial Increment of Pinus sylvestris under Aerotechnogenic Pollution in the Severnaya Dvina River Basin. Lesovedenie [Russian Journal of Forest Science], 2007, no. 2, pp. 45–50.
25. Bates L.S., Waldren R.P., Teare I.D. Rapid Determination of Free Proline for Water-Stress Studies. Plant and Soil, 1973, vol. 39, iss. 1, pp. 205–207. DOI: 10.1007/BF00018060
26. Cook E., Briffa K., Shiyatov S., Mazepa V., Jones P.D. Data Analysis. Methods of Dendrochronology. Ed. by E.R. Cook, L.A. Kairiukstis. Dordrecht, Springer, 1990, pp. 97–162. DOI: 10.1007/978-94-015-7879-0_3
27. Cook E.R. A Time Series Analysis Approach to Tree Ring Standardization. Thesis, PhD in Watershed Management. Tucson, University of Arizona, 1985. 171 p. Available at: https://ltrr.arizona.edu/sites/ltrr.arizona.edu/files/bibliodocs/CookER-Dissertation.pdf (accessed 13.12.19).
28. Crawford R.M.M., Baines M.A. Tolerance of Anoxia and the Metabolism of Ethanol in Tree Roots. New Phytologist, 1977, vol. 79, iss. 3, pp. 519–526. DOI: 10.1111/j.1469-8137.1977.tb02236.x
29. Ferguson C.W. A 7104-Year Annual Tree-Ring Chronology for Bristlecone Pine, Pinus Aristata, from the White Mountains, California. Tree-Ring Bulletin, 1969, vol. 29, no. 3-4, pp. 3–29.
30. Fritts H.C. Tree Rings and Climate. London, Academic Press. 1976. 567 p.
31. Hejnowicz A., Tomaszewski M. Growth Regulators and Wood Formation in Pinus Silvestris. Physiologia Plantarum, 1969, vol. 22, iss. 5, pp. 984–992. DOI: 10.1111/j.1399-3054.1969.tb07456.x
32. Huang B., Johnson J.W. Root Respiration and Carbohydrate Status of Two Wheat Genotypes in Response to Hypoxia. Annals of Botany, 1995, vol. 75, iss. 4, pp. 427–432. DOI: 10.1006/anbo.1995.1041
33. Kontunen-Soppela S. Dehydrins in Scots Pine Tissues: Responses to Annual Rhythm, Low Temperature and Nitrogen. Doctoral Dissertation. Oulu, University of Oulu, 2001. 44 p. Available at: http://jultika.oulu.fi/files/isbn9514259114.pdf (accessed 13.12.19).
34. Krasensky J., Jonak C. Drought, Salt, and Temperature Stress-Induced Metabolic Rearrangements and Regulatory Networks. Journal of Experimental Botany, 2012, vol. 63, iss. 4, pp. 1593–1608. DOI: 10.1093/jxb/e
35. Moffatt B., Ewart V., Eastman A. Cold Comfort: Plant Antifreeze Proteins. Physiologia Plantarum, 2006, vol. 126, iss. 1, pp. 5–16. DOI: 10.1111/j.1399-3054.2006.00618.x
36. Mohr H., Schopfer P. Plant Physiology. Berlin, Springer, 1995. 629 p. DOI: 10.1007/978-3-642-97570-7
37. Oliviusson P., Salaj J., Hakman I. Expression Pattern of Transcripts Encoding Water Channel-Like Proteins in Norway Spruce (Picea abies). Plant Molecular Biology, 2001, vol. 46, iss. 3, pp. 289–299. DOI: 10.1023/A:1010611605142
38. Roohi A., Nazish B., Nabgha-e-Amen, Maleeha M., Waseem S. A Critical Review on Halophytes: Salt Tolerant Plants. Journal of Medicinal Plants Research, 2011, vol. 5 (33), pp. 7108–7118. DOI: 10.5897/JMPRx11.009
39. Sicher R.C., Barnaby J.Y. Impact of Carbon Dioxide Enrichment on the Responses of Maize Leaf Transcripts and Metabolites to Water Stress. Physiologia Plantarum, 2012, vol. 144, iss. 3, pp. 238–253. DOI: 10.1111/j.1399-3054.2011.01555.x
40. Szabados L., Savouré A. Proline: A Multifunctional Amino Acid. Trends in Plant Science, 2010, vol. 15, iss. 2, pp. 89–97. DOI: 10.1016/j.tplants.2009.11.009
41. Tang Z.C., Kozlowski T.T. Ethylene Production and Morphological Adaptation of Woody Plants to Flooding. Canadian Journal of Botany, 1984, vol. 62, no. 8, pp. 1659–1664. DOI: 10.1139/b84-223
42. Topa M.A., McLeod K.W. Responses of Pinus Clausa, Pinus Serotina and Pinus Taeda Seedlings to Anaerobic Solution Culture. I. Changes in Growth and Root Morphology. Physiologia Plantarum, 1986, vol. 68, iss. 3, pp. 523–539. DOI: 10.1111/j.1399-3054.1986.tb03392.x
43. Tripepi R.R., Mitchell C.A. Stem Hypoxia and Root Respiration of Flooded Maple and Birch Seedlings. Physiologia Plantarum, 1984, vol. 60, iss. 4, pp. 567–571. DOI: 10.1111/j.1399-3054.1984.tb04929.x
44. Uggla C., Mellerowicz E.J., Sundberg B. Indole-3-Acetic Acid Controls Cambial Growth in Scots Pine by Positional Signaling. Plant Physiology, 1998, vol. 117, iss. 1, pp. 113–121. DOI: 10.1104/pp.117.1.113
45. Verhoeven A. Sustained Energy Dissipation in Winter Evergreens. New Phytologist, 2014, vol. 201, iss. 1, pp. 57–65. DOI: 10.1111/nph.12466
46. Volger H.G., Heber U. Cryoprotective Leaf Proteins. Biochimica et Biophysica Acta, 1975, vol. 412, iss. 2, pp. 335–349.