Obtaining High-Quality Planting Material of Forest Berry Plants by Clonal Micropropagation for Restoration of Cutover Peatlands

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UDС

634.7

DOI:

https://doi.org/10.37482/0536-1036-2021-2-21-29

Abstract

The results of studies on the improvement of technology for producing highquality planting material of half-high blueberry and Arctic bramble by the method of clonal micropropagation are presented in the current paper. Creation of forest berry plantations in peat extraction areas allows reducing environmental damage and significantly increasing the efficiency of the timber industry. In recent decades, there has been an increasing interest in the creation of forest berry plantations on drained and cutover peatlands in Russia and other countries. It is necessary to use varietal planting material for the successful cultivation of forest berry plants on an industrial scale. Clonal micropropagation is the most effective of the vegetative methods for obtaining planting material, which allows receiving a huge amount of healthy planting material all year round in the conditions of a small laboratory area. Chloride-free ecosterilizer and bleaching agent based on sodium hypochlorite “Belizna” with an exposure of 15 and 20 min showed high efficiency in sterilization of explants of half-high blueberry and Arctic bramble. The highest viability of explants of the studied forest berry crops was observed when sterilized with a 0.1 % mercuric chloride solution and 15 min exposure, and its sharp decrease at 20 min exposure. At the stage of micropropagation, with an increase in the concentration of cytokinin 6-BAP from 0.5 to 1.0 mg/L on the nutrient Woody Plant Medium the number of shoots in regenerated plants of half-high blueberry (Northcountry and Northblue cultivars) and Arctic bramble (Anna and Sofia cultivars) increased. The effect of the concentration of IBA-derived auxin on the number and length of roots of regenerated plants was observed at the in vitro rooting stage.

Authors

Sergey S. Makarov¹, Candidate of Agriculture, Senior Research Scientist;
ResearcherID:AAB-4651-2021, ORCID: https://orcid.org/0000-0003-0564-8888
Irina B. Kuznetsova², Candidate of Agriculture, Assoc. Prof.; ResearcherID: AAB-4568-2021,
ORCID: https://orcid.org/0000-0001-5011-3271
Anton I. Chudetsky¹, Leading Engineer; ResearcherID: H-1210-2019,
ORCID: https://orcid.org/0000-0003-4804-7759
Sergey A. Rodin³, Doctor of Agriculture, Academician of RAS, Deputy Director for Research; ORCID: https://orcid.org/0000-0002-8947-9809

Affiliation

¹Central-European Forest Experiment Station, ARRISMF, prosp. Mira, 134, Kostroma, 156013, Russian Federation; e-mail: makarov_serg44@mail.ru, a.chudetsky@mail.ru
²Kostroma State Agricultural Academy, ul. Uchebnyy gorodok, 34, pos. Karavayevo, Kostroma district, Kostroma region, 156530, Russian Federation; e-mail: sonnereiser@yandex.ru
³All-Russian Research Institute of Silviculture and Mechanization of Forestry, ul. Institutskaya, 15, Pushkino, Moscow region, 141202, Russian Federation

Keywords

Arctic bramble, clonal micropropagation, cultivar, cutover peatlands, growth regulator, half-high blueberry, in vitro

For citation

Makarov S.S., Kuznetsova I.B., Chudetsky A.I., Rodin S.A. Obtaining High-Quality Planting Material of Forest Berry Plants by Clonal Micropropagation for Restoration of Cutover Peatlands. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 2, pp. 21–29. DOI: 10.17238/0536-1036-2021-2-21-29

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