Lactic Acid Synthesis by Fungus Rhizopus oryzae F-1030 on Growth Media Based on Sulphite Liquors

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UDС

663.15

DOI:

10.37482/0536-1036-2020-2-146-158

Abstract

Lactic acid is an industrially important product with an expanding consumer market. However, lactic acid production and isolation methods used at the present time are not effective enough, lead to the formation of large amounts of polluting waste and their recycling is economically unprofitable. At the same time, a half of the worldʼs lactic acid production is carried out by the microbiological method based on the fermentation of such costly sugarcontaining substrates as sucrose, molasses, treacle, sugar syrup, etc. These sugar-containing substrates usage significantly increases the final product cost. In order to solve the conomic and environmental problems of lactic acid production it is necessary to revise the current raw material source and put cheaper and readily available sources of carbohydrates, such as sulphite liquor formed during sulphite pulping, into the lactic acid production. In turn to enhance the economic efficiency of the Russian pulp and paper production it is necessary to use such paper production by-products as sulphite liquor to the fullest extent possible. Sulphite liquor is a chemical complex of inorganic and organic compounds such as monoand oligosaccharides. The article considers the dependence of the output of lactic acid synthesized on the sulphite liquor medium by the fingus R. oryzae F-1030 on the used method of cultivation. In case of the semicontinuous culture method, the culture liquid was replaced with the similar volume of the sterile growth medium with the fungus biomass saving when the sugars in the medium were depleted. In case of the batch culture method, the synthesized  lactic acid was precipitated with calcium hydroxide and the reducing substances recovery in the culture liquid was achieved by adding concentrated sulphite liquor when the sugars in the medium were depleted. The study demonstrates that the largest amount of synthesized lactic acid is obtained when using the semicontinuous method for cultivation of the fingus on the sulphite liquor medium prepared according to the technology recommended in the industry during preparation growth media for yeast cultivation. If it is impossible to carry out a full industrial pre-treatment of sulphite liquor, it is recommended to use the batch culture method for the fungus in order to obtain more synthesized lactic acid.

Authors

L.A. Mingazova, Postgraduate Student; ORCID: https://orcid.org/0000-0003-3289-3977
A.V. Kanarsky, Doctor of Engineering, Prof.; ResearcherID: O-8113-2016,
ORCID: https://orcid.org/0000-0002-3541-2588
E.V. Kryakunova, Candidate of Biology, Assoc. Prof.; ResearcherID: Z-3038-2019, ORCID: https://orcid.org/0000-0003-4563-9847
Z.A. Kanarskaya, Candidate of Engineering, Assoc. Prof.; ResearcherID: AAG-2997-2020, ORCID: https://orcid.org/0000-0002-8194-6185

Affiliation

Kazan National Research Technological University, ul. K. Marksa, 68, Kazan, Republic of Tatarstan, 420015, Russian Federation; e-mail: zleisan1@mail.ru, alb46@mail.ru, oscillatoria@rambler.ru, zosya_kanarskaya@mail.ru

Keywords

sulphite liquor, R. oryzae, semicontinuous culture method, batch culture method, lactic acid

For citation

Mingazova L.A., Kanarsky A.V., Kryakunova E.V., Kanarskaya Z.A. Lactic Acid Synthesis by the Fungus Rhizopus Oryzae F-1030 on Growth Media Based on Sulphite Liquors. Lesnoy Zhurnal [Russian Forestry Journal], 2020, no. 2, pp. 146–158. DOI: 10.37482/0536-1036-2020-2-146-158

References

1. Golomb L.M. Physics and Chemistry of the Technology of Commercial Dyes. Leningrad, Khimiya Publ., 1974. 224 p.
2. Gracheva I.M., Gavrilova N.N., Ivanova L.A. Technology of Microbial Protein Preparations, Amino Acids and Fats. Moscow, Pishchevaya promyshlennostʼ Publ., 1980. 448 p.
3. Grigorʼyeva R.Z., Kurakin M.S. Food Commodity Science. Kemerovo, KemTIPP Publ., 2008. 115 p.
4. Ziganshin D.D., Sirotkin A.S. Features of the Deep and Surface Cultivation of Trichoderma Fungi for Obtaining Biological Products Based on Fungal Cells. Vestnik tekhnologicheskogo universiteta [Herald of Kazan Technological University], 2017, vol. 20, no. 10, pp. 155–158.
5. Korenman I.M. Photometric Analysis. Methods for Determination of Organic Compounds. Moscow, Khimiya Publ., 1970. 343 p.
6. Korneeva O.S., Motina E.A., Yakovleva S.F., Yakovlev A.N. Effect of Culture Conditions on the Growth of Biomass Yarrowia lipolytica – Producing Protein Feed. Vestnik Voronezhskogo gosudarstvennogo universiteta inzhenernykh tekhnologiy [Proceedings of the Voronezh State University of Engineering Technologies], 2016, no. 1, pp. 182–185. DOI: 10.20914/2310-1202-2016-1-182-185
7. Levina E.A., Atykyan N.A., Revin V.V. The Effect of Carbon and Nitrogen Sources on the Production of Cellulases by Fungi Lentinus tigrinus VKM F-3616 D and Trichoderma viride VKM F-1131. Vestnik Voronezhskogo gosudarstvennogo universiteta. Seriya: Khimiya. Biologiya. Farmatsiya [Proceedings of Voronezh State University. Series: Chemistry. Biology. Pharmacy], 2016, no. 1, pp. 85–93.
8. Mukhin V.A. The Influence of SO2 on Wood-Destroying Fungi. Biodiversity and Biological Resources of the Urals and Adjacent Territories: Materials of II International Conference, Orenburg, December 17–18, 2002. Orenburg, OGPU Publ., 2002. 196 p. DOI: 10.13140/RG.2.1.3291.8806
9. Novozhilov E.V. Evaluation of Sulfite Liquors Bioresource as Raw Material for Nutrient Yeast Production. Lesnoy Zhurnal [Russian Forestry Journal], 1999, no. 2-3, pp. 180–188. URL: http://lesnoizhurnal.ru/upload/iblock/ab7/ab7f2300221c7ded546d5483246aa51a.pdf
10. Ovchinnikov Yu.A. Bioorganic Chemistry. Moscow, Prosveshcheniye Publ., 1987. 815 p.
11. Processing of Sulfate and Sulfite Liquors. Ed. by B.D. Bogomolov et al. Moscow, Lesnaya promyshlennostʼ Publ., 1989. 360 p.
12. Smirnov R.E. Production of Sulphite Pulp. Saint Petersburg, SPbGTURP Publ., 2010. 146 p. 
13. Chemistry of Petroleum Hydrocarbons. Ed. by B.T. Brooks et al. Moscow, Gostoptekhizdat Publ., 1959, vol. 3. 583 p.
14. Sharkov V.I., Sapotnitskiy S.A., Dmitriyeva O.A., Tumanov I.F. Technology of Hydrolysis Production. Moscow, Lesnaya promyshlennostʼ Publ., 1973. 407 p.
15. Shinkarev S.M., Samujlenko A.I., Neminuschiy L.A., Skotnikova T.A., Pavlenko I.V., Rubtsova G.N., Kanarskiy A.V., Mingazova L.A. Improvement of Microbiological Synthesis of Lactic Acid. Vestnik tekhnologicheskogo universiteta [Herald of Kazan Technological University], 2017, vol. 20, no. 18, pp. 165–170.
16. Abd Alsaheb R.A., Aladdin A., Othman N.Z., Abd Malek R., Mei Leng O., Aziz R. et al. Lactic Acid Applications in Pharmaceutical and Cosmeceutical Industries. Journal of Chemical and Pharmaceutical Research, 2015, vol. 7, iss. 10, pp. 729–735.
17. Ghaffar T., Irshad M., Anwar Z., Aqil T., Zulifqar Z., Tariq A. et al. Recent Trends in Lactic Acid Biotechnology: A Brief Review on Production to Purification. Journal of Radiation Research and Applied Sciences, 2014, vol. 7, iss. 2, pp. 222–229. DOI: 10.1016/j.jrras.2014.03.002
18. Khalid K. An Overview of Lactic Acid Bacteria. International Journal of Biosciences, 2011, vol. 1, no. 3, pp. 1–13.
19. Komesu A., Oliveira J.A.R.d., Martins L.H.d.S., Wolf Maciel M.R., Maciel Filho R. Lactic Acid Production to Purification: A Review. BioResources, 2017, vol. 12(2), pp. 4364–4383.
20. Martineza F.A.C., Balciunas E.M., Salgado J.M., Gonzalez J.M.D., Converti A., Oliveira R.P.D.S. Lactic Acid Properties, Applications and Production: A Review. Trends in Food Science & Technology, 2013, vol. 30, iss. 1, pp. 70–83. DOI: 10.1016/j.tifs.2012.11.007
21. Rattanachaikunsopon P., Phumkhachorn P. Lactic Acid Bacteria: Their Antimicrobial Compounds and Their Uses in Food Production. Annals of Biological Research, 2010, vol. 1(4), pp. 218–228.
22. Rhee S.J., Lee J.-E., Lee C.-H. Importance of Lactic Acid Bacteria in Asian Fermented Foods. Microbial Cell Factories, 2011, vol. 10, art. S5. DOI: 10.1186/1475-2859-10-S1-S5
23. Simion A.I., Grigoras C.G., Bardaşu L.E., Dabija A. Modelling of the Thermophysical Lactic Acid Aqueous Solutions. Density and Viscosity. Food and Environment Safety, 2012, vol. XI, iss. 4, pp. 49–58.
24. Wu X., Jiang S., Liu M., Pan L., Zheng Z., Luo S. Production of L-Lactic Acid by Rhizopus Oryzae Using Semicontinuous Fermentation in Bioreactor. Journal of Industrial Microbiology & Biotechnology, 2011, vol. 38, pp. 565–571. DOI: 10.1007/s10295-010-0804-8
25. Yuwa-amornpitak T., Chookietwattana K. L-Lactic Acid Production from Cassava Starch by Thermotolerant Rhizopus microsporus LTH23. Journal of Biological Sciences, 2014, vol. 14, iss. 4. pp. 284–291. DOI: 10.3923/jbs.2014.284.291 

Received on August 26, 2019