Bioconversion of the Cellulose-containing Materials in the Arctic Region Conditions

Complete text of the article:

Download article (pdf, 0.9MB )

UDС

504.064.2

DOI:

10.17238/issn0536- 1036.2019.4.179

Abstract

The regions of the Far North and the Arctic (the territory of which includes the Arkhangelsk region) are characterized by extreme natural and climatic factors: low temperatures in winter, a large daily temperature drop, frequent snowstorms in winter and rains in summer. These conditions significantly affect the enzymatic activity of soil microorganisms. The aim of the work is to assess the effect of the enzymatic treatment temperature on the initial stages of biodegradation of the cellulose – containing materials. The study used samples of cardboard and paper products: newspaper paper with color printing, container cardboard and white office paper. Bacterial cellulose was obtained by cultivating a symbiosis of the genus Acetobacter bacteria and yeast on a glucose medium in static conditions at 25 °C. to simulate biodegradation, a laboratory enzyme preparation was used, produced by a strain of a microscopic fungus. The activity of soil cellulases initiating bioconversion of cellulose-containing materials in soils of municipal solid waste storage areas of the Arkhangelsk region was low and amounted to less than 10 μg of glucose/10 g of soil per 48 hours of exposure. The determining factor for the bioconversion process in the Arctic region is the soil temperature. It is shown that while a decrease in temperature to 5...15 °C, the bioconversion rate of cellulose-containing materials of plant origin is reduced by 2...8 times compared to the temperature of 30...50 °C, which is optimal for many enzymatic processes. It was found that bioconversion of bacterial cellulose to glucose is weakly dependent on the temperature of enzymatic treatment in the range of 5...15 °C and averages 20% for 1 day.

Authors

K.S. Bolotova¹, Candidate of Engineering, Assoc. Prof.; ResearcherID: G-1760-2019, ORCID: 0000-0002-7916-2410
O.V. Travina², Intern-researcher; ResearcherID: T-2952-2018, ORCID: 0000-0001-7049-9271
A.S. Aksenov¹, Candidate of Engineering, Head of the department; ResearcherID: C-7289-2015, ORCID: 0000-0003-1013-1357
M.V. Emelyanova¹, Candidate of Engineering, Assoc. Prof.; ORCID: 0000-0001-6600-6526
V.A. Rudakova¹, Candidate of Engineering, Assoc. Prof.; ORCID: 0000-0002-3447-4226
A.V. Kanarskiy³, Doctor of Engineering, Prof.; ResearcherID: O-8113-2016, ORCID: 0000-0002-3541-2588

Affiliation

¹Northern (Arctic) Federal University named after M.V. Lomonosov, 17 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163002, Russian Federation; e-mail: k.bolotova@narfu.ru, a.s.aksenov@narfu.ru, m.emelyanova@narfu.ru, v.rudakova@narfu.ru
²Federal State Budgetary Institution of Science of the Federal Research Centre for Arctic Integrated Research named after academician N.P. Laverov, Russian Academy of Sciences, 23 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163000, Russian Federation; e-mail: travina.oksana136@yandex.ru
³Kazan National Research Technological University, 68 K. Marx str., Kazan, 420015, Russian Federation; e-mail: alb46@mail.ru

Keywords

biodegradation of cellulose-containing materials, bacterial cellulose, enzymatic hydrolysis, cellulose

For citation

Bolotova K.S., Travina O.V., Aksenov A.S., Emelyanova M.V., Rudakova V.A., Kanarskiy A.V. Bioconversion of Cellulose-containing Materials in the Arctic Region Conditions. Lesnoy Zhurnal [Forestry Journal], 2019, no. 4, pp. 179–186. DOI: 10.17238/issn0536- 1036.2019.4.179

References

1. Alimova F.K., Tuhbatova D.I., Tazetdinova D.I. Methods for Determination of the Hydrolases of Soils and Soil Microorganisms: Study Guide. Kazan, Kazan University Publ., 2010, 67 p.
2. Bolotova K.S., Novozhilov E.V. Application of Enzymatic Technologies to Improve the Environmental Safety of Pulp and Paper Production. Khimija rastitel’nogo syrya [Chemistry of the Vegetable Stock], 2015, no. 3, pp 5–23. DOI: https://doi.org/10.14258/jcprm.201503575
3. Bolotova K.S., Chukhchin D.G., Mayer L.V., Gurya A.G. Morphological features of the fibrous structure of plant and bacterial cellulose. Lesnoy Zhurnal [Forest journal], 2016, no. 6, pp. 153–165. DOI: 10.17238/issn0536-1036.2016.6.153
4. Obolenskaya A.V., Elnitskaya Z.P., Leonovich A.A. Laboratory Works on Chemistry of Wood and Cellulose. Moscow, Ecology Publ., 1991. 320 p.
5. Technology of Pulp and Paper Production. In 3 vol. Vol. 1 – Raw Materials and Semi-finished Products, Part 2 – Pproduction of Semi-finished Products. St. Petersburg, Polytechnic Publ., 2003. 633 p.
6. Titova V.I., Kozlov A.V. Methods of Evaluation of the Soil Microbiocenosis Functioning, Involved in the Transformation of an Organic Matter. Nizhny Novgorod, Nizhny Novgorod State Agricultural Academy Publ., 2012. 64 p.
7. Chekushina A.V. The Cellulolytic Enzymatic Preparations Based on the Hydrolytic Activity-enhanced Trichderma, Penicillium and Myceliophtora Fungi: Cand. Eng. Sci. Diss. (abstract). Moscow, 2013. 23 p.
8. Chertovskaya V.G. Spruce Forests of the European Part of the USSR. Moscow, Forest Industry Publ., 1978. 176 p.
9. Chatterjee S., Sharma S., Prasad R.K., Datta S., Dubey D., K Meghvansi M., G Vairale M., Veer V. Cellulose Enzyme Based Biodegradation of Cellulosic Materials: An Overview (2015) South Asian J Exp. Biol., 5 (6), pp. 271–282, DOI: https://doi.org/10.1007/978-981-10-7485-1_14
10. Kadere T.T., Miyamoto T., Oniang`o R.K., Kutima P.M., Njoroge S.M., Isolation and Identification of the Genera Acetobacter and Gluconobacter in Coconut Toddy (Mnazi). African J Biotechnol, 2008. Vol. 7 (16). Pp. 2963–2971.
11. Kawecki M., Krystynowicz A., Wysota K., Czaja W., Sakiel S. et al. Bacterial Cellulose Biosynthesis, Properties and Applications. Proceedings of the International Review Conference Biotechnology, Vienna, Austria, 2004. Pp. 14–18.
12. Lynd L.R, Weimer P.J., van Zyl W.H., Pretorius I.S. Microbial Cellulose Utilization: Fundamentals and Biotechnology Microbiol Mol Biol Rev, 2002. Vol. 66(3). Pp. 506–577. DOI: 10.1128/MMBR.66.3.506-577.2002
13. Ojumu T., Solomon V., Bamidele O., Betiku E., Layokun S.K, et al. Cellulose Production by Aspergillus Flavus Linn Isolate NSPR 101 Fermented in Sawdust, Bagasse and Corncob. African J Biotechnol, 2003. Vol. 2. Pp. 150–152.
14. Perez J., Munoz-Dorado J., Rubia T. de la, Martınez J. Biodegradation and Biological Treatments of Cellulose, Hemicelluloses and Lignin: an overview. International Microbiology, 2002. Vol. 5. Isue 2. Pp.53–63, DOI: https://doi.org/10.1007/s10123-002-0062-3
15. Wierzba S., Nabrdalik M. Biodegradation of Cellulose in Bottom Sediments of Turawa lake. Physicochemical Problems of Mineral Processing, 2007. Vol. 41 (1). Pp. 227–235.

Received on September 19, 2018