Bacterial Endophytes of Poplar

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UDС

579.64:633.878.32

DOI:

10.17238/issn0536-1036.2018.4.161

Abstract

Biotechnologies provide an opportunity to solve global problems of sustainable development of economic activity. The study of biodiversity of plant endophytic bacteria allows creating new effective biologics for adaptive plant growing. The goal of research is to identify endophyte poplar bacteria in the North (Arkhangelsk). Samples were selected at the beginning of October in the crowned trees of laurel-leafed poplar and fragrant poplar, which had no external signs of pathology and stem rot. The studies were based on the characteristics of isolates obtained from the internal parts of the central wood of poplar tree stems after surface sterilization. Sawdust was examined by the fouling method. Meatpeptone agar was used as the nutrient medium. To determine the biochemical activity of pure bacterial cultures, Hiss's differential diagnostic media were used, including maltose, glucose, sucrose, sorbitol, mannitol, and lactose. For bacterial isolates, identification was carried out by sequencing fragments of the 16S рРНК gene and comparing the resulting nucleotide sequences with those homologous from the NCBI's GenBank database. When the sawdust was sowed in the nutrient medium, fouling with bacterial mass was noted. The sawdust of laurel-leafed poplar overgrew more intensively in comparison with fragrant poplar. Four strains of bacteria (Bacillus pumilus, Lysinibacillus fusiformis, Staphylococcus pasteuri, Brenneria salicis) were isolated from the laurel-leafed poplar wood, and 2 strains were isolated from fragrant poplar (Bacillus safensis, Brenneria populi). The article presents the morphological and biochemical characteristics of bacteria. Most bacteria successfully use the carbon components of the Hiss’ media, with the exception of sorbitol. The ability to metabolize lactose and mannitol is particularly unstable. The widest range of carbohydrates is fermented by strains belonging to the genus Brenneria.

Authors

A.S. Aksenov¹, Candidate of Engineering Sciences, Associate Professor
N.A. Kuz’mina², Research Assistant

Affiliation

¹Northern (Arctic) Federal University named after M.V. Lomonosov, Naberezhnaya Severnoy Dviny, 17, Arkhangelsk, 163002, Russian Federation; e-mail: a.s.aksenov@narfu.ru
²Institute of Biogeography and Genetic Resources, N. Laverov Federal Center for Integrated Arctic Research, Russian Academy of Sciences, Naberezhnaya Severnoy Dviny, 23, Arkhangelsk, 163000, Russian Federation

Keywords

bacterial endophyte, laurel-leafed poplar, fragrant poplar, wood

For citation

Aksenov A.S., Kuz’mina N.A. Bacterial Endophytes of Poplar. Lesnoy zhurnal [Forestry journal], 2018, no. 4, pp. 161–166. DOI: 10.17238/issn0536-1036.2018.4.161

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Received on May 03, 2018