Physiological State Evaluation of Representatives of the Genus Larch (Larix Mill.) in the Nizhny Novgorod Region

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UDС

630*232.12:582.475.2

DOI:

10.17238/issn0536-1036.2018.1.9

Abstract

The formation of newly created plantations assumes the possibility of expanding their species composition and biological diversity of forest vegetation. The introduction has long been recognized as a successful tool for replenishing the local flora with promising species. Stability of species in new conditions is directly related to their physiological characteristics. The practice of introducing representatives of the genus larch confirms wide biological possibilities, high stability and productivity of plantations created from them. The indicators of seasonal changes of the storage compound content in the tissues of one-year shoots are an important characteristic of the physiological state of woody plants as native representatives of the genus larch and its exotics. The goal of research is to identify the interspecific and individual phenotypic variability of various species of larch under the conditions of introduction according to the indices of their physiological state. The object of the study is the collection of larch plantations in the territory of the dendrological complex “Yavleika” in the Sergachskiy interdistrict forestry of the Nizhny Novgorod region. We investigate the presence of starch (staining reaction to Lugol's solution) and fats (Sudan III reaction) in the tissues of one-year shoots of three species of larch. The content of these components is assessed in terms of the developed scales. The primary sampling unit in the histochemical experiment is a one-time fixation of the amount of the analyzed matter in the shoot's recording tissue. Visual reading of values is performed on temporary preparations of transverse sections from the middle part of the annual increment. Sections after staining and fixation are analyzed using a micrometer “Mikmed-2”. Common methods are used for the statistical and variance analyses. We have determined the degree of genotypic conditioning of characteristics of the physiological state of plants, the influence of environmental factors on the formation of phenotypic differences between them. The species of a genus larch essentially differ in ability to form lipoid stocks in one-year shoots of different phenological phases. The highest content of fats in March was observed in Sukachev’s larch (18.33 points) and Dahurian larch (17.50 points), Siberian larch had the less indicator (7.84 points). During the period of active shoot growth (in the end of May), the content of fats in their tissues decreased in the studied species, the differences between them were equalized. Considered species of the genus larch differ significantly by the ability to form starch stocks in one-year shoots in different phenological phases. The highest content of starch (in the end of May) was characteristic of Dahurian larch, the lowest − for Sukachev’s larch. The ranges of values of the test characters in different species of larch are not the same and vary according to the phenological phases. Phenotypic differences in the studied indicators are determined both by species and by external factors. The environmental effect on the formation of stocks of fatlike substances and starch depends on the phenological state of plants.

Authors

V.P. Besschetnov, Doctor of Biological Sciences, Professor
N.N. Besschetnova, Doctor of Agricultural Sciences, Associate Professor
A.O. Esichev, Postgraduate Student

Affiliation

Nizhny Novgorod State Agricultural Academy, pr. Gagarina, 97, Nizhny Novgorod, 603107, Russian Federation; е-mail: lesfak@bk.ru, besschetnova1966@mail.ru, andrey.esichev@mail.ru

Keywords

genus larch, storage compound, fats, starch, introduction, variability, physiological state, one-year shoot, genotypic conditioning, adaptation

For citation

Besschetnov V.P., Besschetnova N.N., Esichev A.O. Physiological State Evaluation of Representatives of the Genus Larch (Larix Mill.) in the Nizhny Novgorod Region. Lesnoy zhurnal [Forestry journal], 2018, no. 1, pp. 9–17. DOI: 10.17238/issn0536-1036.2018.1.9

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Received on May 17, 2017