Stimulation of Germination and Germination Energy of Scots Pine Seeds with Biochemical Preparations. P. 189–200

Complete text of the article:

Download article (pdf, 0.9MB )

UDС

630.232

DOI:

10.37482/0536-1036-2025-6-189-200

Abstract

Currently, the task of obtaining high-quality planting stock used in reforestation remains urgent. Stimulating preparations are used to activate germination and seed germination energy. The aim of the work has been to study the effectiveness of using various stimulating preparations on the sowing qualities of Scots pine (Pinus sylvestris L.) seeds. The objects of the study have been Pinus sylvestris L. seeds soaked in solutions of preparations with a purported stimulating effect. The following preparations have been applied: technical lingosulfonates, birch bark extracts, filtrate obtained by separating suberin from birch bark, Flavobacterin, Mizorin, condensate formed during the drying of sawn timber, and ash from the Eyjafjallajökull Volcano. In the experiments, several time options for soaking seeds have been adopted: 6, 12 and 24 hours. The highest germination and germination energy have been observed in seeds left for 24 hours. The indicators of the seed grain soaked for 6 and 12 hours have also increased compared to the control sample of dry seeds. The best germination has been observed in seeds treated with solutions of nitrosated and chlorinated lingosulfonates at a concentration of 10 % for 24 hours (95 %), the highest germination energy (86 %) has been observed in seeds left for 24 hours in an extract of birch bast fraction less than 1 mm (concentration 1:10,000) and filtrate from the separation of suberin from birch bark (concentration 1:10,000). In the control option, the seed germination has been equal to 80 %.

Authors

Anna A. Drochkova, Postgraduate Student; ResearcherID: H-1869-2019, ORCID: https://orcid.org/0000-0003-3503-5767
Natal’ya R. Sungurova, Doctor of Agriculture, Assoc. Prof.; ResearcherID: H-1847-2019, ORCID: https://orcid.org/0000-0002-8464-4596
Denis N. Klevtsov, Doctor of Biology; ResearcherID: A-7791-2019, ORCID: https://orcid.org/0000-0001-6902157X

Affiliation

Northern (Arctic) Federal University named after M.V. Lomonosov, Naberezhnaya Severnoy Dviny, 17, Arkhangelsk, 163002, Russian Federation; annadrochkova@gmail.com, n.sungurova@narfu.ru, d.klevtsov@narfu.ru

Keywords

Pinus sylvestris L., pine seeds, growth stimulant, sowing qualities

For citation

Drochkova A.A., Sungurova N.R., Klevtsov D.N. Stimulation of Germination and Germination Energy of Scots Pine Seeds with Biochemical Preparations. Lesnoy Zhurnal = Russian Forestry Journal, 2025, no. 6, pp. 189–200. (In Russ.). https://doi.org/10.37482/0536-1036-2025-6-189-200

References

1. Babich N.A., Barabin A.I., Tutygin G.S. The State and Problems of Reforestation in the European North. Collection of Scientific Papers. Arkhangelsk, 1999, pp. 4–12. (In Russ.).

2. Belyaev V.V. The State and Main Directions for Increasing the Efficiency of Reforestation in the European North of Russia. Vestnik Pomorskogo Universiteta = Vestnik of Pomor University, 2001, iss. 2, pp. 45–50. (In Russ.).

3. Drochkova A.A. Analysis of the Effect of Lignosulfonates on the Germination and Germination Energy of Pinus sylvestris L. Seeds. Papaninskie chteniya = The Papanin Readings: Articles by Participants of the International Youth Scientific Conference. Arkhangelsk, Northern (Arctic) Federal University named after M.V. Lomonosov Publ., 2017, pp. 52–55. (In Russ.).

4. Drochkova A.A. Analysis of the effects of condensate formed during drying of lumber on germination and germination energy of Pinus sylvestris L. seeds. Aktu al’nye problemy razvitiya lesnogo kompleksa: Proceedings of the XVIII International Scientific and Technical Conference. Vologda, Vologda State University Publ., 2020, pp. 31–32. (In Russ.).

5. Drochkova A.A. Analysis of the Influence of Eyjafjallajökull Volcano Ash on the Main Quality Indicators of Pinus sylvestris L. Seeds. Fundamental’nye i prikladnye nauchnye issledovaniya: aktual’nye voprosy, dostizheniya i innovatsii: Collection of Articles of the XLIV International Scientific and Practical Conference. Penza, Nauka i prosveshchenie Publ., 2021, pp. 137–139. (In Russ.).

6. Drochkova A.A. The Influence of Soaking Time of Scots Pine Seeds on Their Quality Indicators. Fundamental’naya i prikladnaya nauka: aktual’nye voprosy teorii i praktiki: Collection of Articles of the III International Scientific and Practical Conference. Penza, Nauka I prosveshchenie Publ., 2023, pp. 99–101. (In Russ.).

7. Egorova A.A., Babich N.A. The Effect of “Flavobacterin” on the Germination and Germination Energy of Scots Pine Seeds. Ekologichesikie problemy Arktiki i severnykh territorij: Interuniversity Collection of Scientific Papers, iss. 15. Arkhangelsk, Northern (Arctic) Federal University named after M.V. Lomonosov Publ., 2012, pp. 89–91. (In Russ.).

8. Kunavin A.A., Kutakova N.A., Drochkova A.A., Badogina A.I. Measurement of Growth-Stimulating Properties of Birch Bark Bast Extracts. Aktual’nye problemy metrologicheskogo obespecheniya nauchno-prakticheskoj deyatel’nosti: Proceedings of the All-Russian Scientific and Technical Conference of Young Scientists, Postraduate Students and Students. Arkhangelsk, Northern (Arctic) Federal University named after M.V. Lomonosov Publ., 2016, pp. 70–74. (In Russ.).

9. Ostroshenko V.Yu., Ostroshenko L.Yu. Influence of Growth Stimulants on Seed Germination and Seedlings Growth of Scots Pine (Pinus sylvestris L.). Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 4, pp. 93–104. (In Russ.). https://doi.org/10.37482/0536-1036-2023-4-93-104

10. Pentelkin S.K. Results of the Study of Growth Stimulants and Polymers in Forestry over the Past 20 Years. Lesokhozyajstvennaya informatsiya = Forestry Information, 2003, no. 10, pp. 34–53. (In Russ.).

11. Rodin A.R., Popova N.Ya. Prospects for the Use of Environmentally Friendly Preparations in Forest Nurseries. Lesnoy Zhurnal = Russian Forestry Journal, 1991, no. 6, pp. 3–7. (In Russ.).

12. Rodin A.R., Popova N.Ya., Kandyba E.V. Highly Effective Preparations for Nurseries. Lesnoe khozyajstvo, 1997, no. 1, pp. 28–30. (In Russ.).

13. Runova E.M., Denisenko A.V. The Influence of Growth-Promoting Preparations on the Germination Rates of Scots Pine (Pinus sylvestris L.) Seeds. Aktual’nye problemy lesnogo kompleksa, 2024, no. 65, pp. 125–128. (In Russ.).

14. Dictionary and Reference Book of the Taiga Forestry Specialist: Reference Book. Arkhangelsk, Arkhangelsk State Technical University Publ., 2005. 252 p. (In Russ.).

15. Sungurova N.R., Drochkova A.A., Gayevskiy N.P., Volykhina N.V., Babich N.A. Wood Drying Condensate as Pinus sylvestris L. Seeds Germination Activator. Lesnoy vestnik = Forestry Bulletin, 2022, vol. 26, no. 4, pp. 39–45. (In Russ.). https://doi.org/10.18698/2542-1468-2022-4-39-45

16. Tyukavina O.N., Demina N.A. Biologically Active Substances for Stimulating the Growth of Coniferous Seedlings. Vestnik Buryatskoj gosudarstvennoj sel’skokhozyajstvennoj akademii imeni V.R. Filippova = Vestnik of Buryat State Academy of Agriculture named after V. Philippov, 2023, no. 1(70), pp. 122–133. (In Russ.). https://doi.org/10.34655/bgsha.2023.70.1.015

17. Barber J.J. Recent Advances in Planting Techniques. Forest Farmer, 1972, vol. 32. 26 p.

18. Calvo P., Nelson L., Kloepper J.W. Agricultural Uses of Plant Biostimulants. Plant and Soil, 2014, vol. 383, pp. 3–41. https://doi.org/10.1007/s11104-014-2131-8

19. Davis A.S., Jacobs D.F. Quantifying Root System Quality of Nursery Seedlings and Relationship to Outplanting Performance. New Forests, 2005, vol. 30, pp. 295–311. https://doi.org/10.1007/s11056-005-7480-y

20. Grossnickle S.C. Why Seedlings Survive: Influence of Plant Attributes. New Forests, 2012, vol. 43, pp. 711–738. https://doi.org/10.1007/s11056-012-9336-6

21. Grossnickle S.C., MacDonald J.E. Why Seedlings Grow: Influence of Plant Attributes. New Forests, 2018, vol. 49, pp. 1–34. https://doi.org/10.1007/s11056-017-9606-4

22. Hoffmann F. Untersuchungen zur Stickstoffernahrung junger Koniferen. Archiv fur Forstvesen, 1966, no. 10, pp. 1093–1103. (In Germ.).

23. Louder R. Volcanic Ash May Be Helping Vegetation Growth. Reykjavik, Grapevine Publ., 2010. 24 p.

24. Rikala R. Seedling Production for Reforestation. Forest Regeneration in the Northern Parts of Europe: Proceeding of the Finnish-Russian Forest Regeneration Seminar in Vuokatti, Finland, 2000, pp. 127–140.

25. Salaš P., Sasková H., Mokričková J., Litschmann T. Evaluation of Different Types of Rooting Stimulators. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2013, vol. 60, iss. 8, pp. 217–228. https://doi.org/10.11118/actaun201260080217

26. Shchukin R.A., Bogdanov O.E., Zavoloka I.P., Ryazanov G.S., Kruglov N.M. Biotechnological Basis for Application of Growth Regulators for Rooting of Green Cuttings of Trees and Shrubs in a Greenhouse with a Misting System. BIO Web of Conferences, 2020, vol. 23, art. no. 01009. https://doi.org/10.1051/bioconf/20202301009