Enzymatic Activity of Filamentous Fungus Trichoderma reesei M18 in Culture in the Nutrient Solution Based on Peat Lignocellulose

Complete text of the article:

Download article (pdf, 0.4MB )

UDС

552.577:676.01: 577.152.3

DOI:

10.17238/issn0536-1036.2016.6.142

Abstract

Integrated processing of peat is an important economic issue. The authors have obtained the iron chelates with humic acids by the electrochemical synthesis method. The acids are recommended for use as a feed additive. Due to the formation of the secondary resource – lignocellulose we have encountered a problem of the technology of its processing into the cost-effective products. Lignocellulose contains a large amount of pulp that makes it an attractive material for the biotechnological processing by microorganisms. However, lig-nocellulose contains lignin, which prevents the enzymatic hydrolysis of cellulose into simple sugars. This fact demands the ways of its pretreatment. Lignocellulose can be used as a nutrient substrate for the cultivation of filamentous fungi of the Trichoderma genus, since they are characterized by the expression of xylanase and cellulase activities, which promote the enzymatic hydrolysis of lignocellulose. The work objective is a definition of the enzyme activity in culture of the strain of filamentous fungus Trichoderma reesei M18 in the nutrient solution based on peat lignocellulose. To enhance the enzymatic activity of filamentous fungus we injected a multi-enzymic complex comprising cellulase and xy-lanase enzymes and reducing agents into the nutrient solution with peat lignocellulose in a solid phase. Lignocellulose was also treated with sodium bisulfite in the experiments; lignin was dissolved and cellulose became more accessible for assimilating by fungus. The expediency of filamentous fungus Trichoderma reesei M18 cultivation in the nutrient solution based on peat lignocellulose, pre-treated with sodium bisulfite, is established. The introduction of multi-enzymic complexes, containing cellulase and xylanase, into the nutrient solution based on lignocellulose, treated with sodium bisulfite, improves the en-zyme activity of Trichoderma reesei M18, which increases the protein and biomass syn-thesis. The pretreatment of peat lignocellulose by sodium bisulfite is recommended for the bioconversion by mycelial fungus Trichoderma reesei M18 with adsorbent of mycotoxins obtaining from biomass. The biomass of filamentous fungi Trichoderma reesei M18, grown on peat lignocellulose, is used to produce the adsorbents of mycotoxins, which successfully have been tested in vivo.

Authors

S.V. Gavrilov1, Postgraduate Student A.V. Kanarskiy1, Doctor of Engineering Sciences, Professor E.V. Skvortsov2, Сandidate of Biological Sciences Yu.V. Sevast'yanova3, Candidate of Engineering Sciences, Associate Professor

Affiliation

1Kazan National Research Technological University, ul. K. Marksa, 72, Kazan, 420015, Russia Federation; e-mail: ser-gavr@mail.ru 2Kazan Federal University, Kremlyovskaya str., 18, Kazan, 420000, Russian Federation; e-mail: eskvortsov@rambler.ru
3Northern (Arctic) Federal University named after M.V. Lomonosov, Naberezhnaya Severnoy Dviny, 17, Arkhangelsk, 163002, Russia Federation; e-mail: j.sevastyanova@narfu.ru

Keywords

peat, lignocellulose, sodium bisulfite treatment, xylanase, cellulase, Trichoderma reesei M18 cultivation.

For citation

Gavrilov S.V., Kanarskiy A.V., Skvortsov E.V., Sevast’yanova Yu.V. Enzy-matic Activity of Filamentous Fungus Trichoderma reesei M18 in Culture in the Nutrient Solution Based on Peat Lignocellulose. Lesnoy zhurnal (Forestry journal), 2016, no. 6, pp. 142–152. DOI: 10.17238/issn0536-1036.2016.6.142

References

1. Gavrilov S.V., Kanarskaya Z.A. Adsorbtsionnye svoystva torfa i produktov ego pererabotki [The Adsorption Properties of Peat and Its Derivative Products]. Vestnik Ka-zanskogo tekhnologicheskogo universiteta, 2015, vol. 18, no. 2, pp. 422–427.
2. Gavrilov S.V., Kanarskiy A.V., Sidorov Yu.D. Poluchenie khelatnykh soedi-neniy zheleza s guminovymi kislotami metodom elektrokhimicheskogo sinteza [Preparation of Iron Chelating with Humic Acids by Electrochemical Synthesis]. Vestnik Kazanskogo tekhnologicheskogo universiteta, 2012, no. 9(15), pp. 165–168.
3. Grekova A.A., Mal'tsev A.N., Dremza I.K. Protektivnoe deystvie suspenzii torfa pri mikotoksikozakh [The Protective Effect of Peat Suspension on Mycotoxicoses]. Sb. nauch. tr. Stavropol'skogo NII zhivotnovodstva i kormoproizvodstva [Proc. Stavropol Re-search Institute of Livestock and Fodder Production], 2011, no. 4–1(1), pp. 136–140.
4. Morozova Yu.A., Skvortsov E.V., Alimova F.K., Kanarskiy A.V. Biosintez ksi-lanaz i tsellyulaz gribami roda Trichoderma na poslespirtovoy barde [The Biosynthesis of Xylanase and Cellulase by Fungi of Trichoderma Genus on DDGS]. Vestnik Kazanskogo tekhnologicheskogo universiteta, 2012, vol. 15, no. 19, pp. 120–122.
5. Nemashkalov V.A., Koshelev A.V., Okunev O.N. Biosintez karbogidraz griba Penicillium verruculosum pri kul'tivirovanii na razlichnykh tsellyulozosoderzhashchikh sub-stratakh [Carbohydrases Biosynthesis of Penicillium verruculosum Fungus when Cultured on Different Cellulosic Substrates]. Tez. 13-y Mezhdunar. Pushchinskoy shk.-konf. mo-lodykh uchenykh “Biologiya – nauka XX veka” [Proc. 13th Intern. Pushchino School-Conf. of Young Scientists “Biology is the Science of the 20th Century”]. Pushchino, 2009, p. 174.
6. Khalimova L.Kh., Sharaeva A.V., Sokolova S.Yu., Petukhova N.I., Zorin V.V. Issledovanie vliyaniya induktorov na sintez lakkaz mitselial'nymi gribami [The Effect of Inductors on the Synthesis of Laccases by Filamentous Fungi]. Bashkirskiy khimicheskiy zhurnal [Bashkir Chemical Journal], 2010, no. 5(17), pp. 46–49.
7. Chukhareva N.V. Issledovanie gruppovogo sostava torfov mestorozhdeniy Tomskoy oblasti [The Study of the Peat Group Composition of Deposits of Tomsk Region]. Vestnik Krasnoyarskogo gosudarstvennogo agrarnogo universiteta [The Bulletin of KrasGAU], 2013, no. 7, pp. 65–71.
8. Burba P., Jakubowski B., Kuckuk R., Kullmer K., Heumann K.G. Characteriza-tion of Aquatic Humic Substances and Their Metal Complexes by Immobilized Metal-Chelate Affinity Chromatography on Iron (III) – Loaded Ion Exchangers. Fresenius' J. of Analytical Chemistry, 2000, vol. 7(386), pp. 689–696.
9. Camassola M., Dillon A.J.P. Production of Cellulases and Hemicellulases by Penicillium Echinulatum Grown on Pretreated Sugar Cane Bagasse and Wheat Bran in Sol-id-State Fermentation. Journal of Applied Microbiology, 2007, no. 103(6), pp. 2196–2204.
10. Islam K.M.S., Schuhmacher A., Gropp J.M. Humic Acid Substances in Animal Agriculture. Pakistan Journal of Nutrition, 2005, vol. 4(3), pp. 126–134.
11. Measurement of Cellulase Activities. Ed. by T.K. Ghose. Pure & Appl. Chem., 1987, no. 2, pp. 257–268.
12. Mezes M., Erd?lyi M., Balogh K. Deposition of Organic Trace Metal Complex-es as Feed Additives in Farm Animals. Eur. Chem. Bull., 2012, vol. 1(10), pp. 410–413.
13. K?nig J., Grasser R., Pikor H., Vogel K. Determination of Xylanase, ?-Glucanase, and Cellulase Activity. Anal. Bioanal. Chem., 2002, vol. 374, no. 1, pp. 80–87.
14. Sloneker J.H. Determination of Cellulose and Apparent Hemicellulose in Plant Tissue by Gas-Liquid Chromatography. Analytical Biochemistry, 1971, no. 2(43), pp. 539–546.
Received on June 08, 2016