Clonal Micropropagation Technology of Relict Gymnosperms Sequoia sempervirens (D. Don) Endl.. P. 73-87

Complete text of the article:

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UDС

576.316

DOI:

10.37482/0536-1036-2026-2-73-87

Abstract

Sequoia sempervirens (D. Don) Endl. is the tallest relict plants capable of accumulating unique secondary metabolites that can be used in pharmacognosy. Given the documented reproductive vulnerability of the coast redwood, biotechnological methods for establishing in vitro genetic banks and bioresource collections are increasingly relevant for the preservation of its gene pool. This study utilized nodal segments of S. sempervirens harvested from a donor tree maintained in the stock greenhouse of the N.V. Tsitsin Main Botanical Garden of the Russian Academy of Sciences (Moscow). The research investigates the effects of mineral and hormonal nutrient media compositions, as well as the explant’s original position on the shoot, on in vitro propagation efficiency. Stepwise sterilization using a 0.1 % (w/v) mercuric chloride solution for 18 min resulted in a successful sterile culture rate exceeding 80 %. Findings indicate that explants should initially be cultured on a hormone-free Murashige and Skoog (MS) basal medium, followed by a proliferation medium supplemented with either 2 mg/L 2,4-D + 0.5 mg/LBAP + 0.5 mg/LNAA or 2 mg/L 2,4-D + 2 mg/L 2iP for effective multiplication. For rooting, it is advisable to transfer regenerants to a medium containing 2 mg/L 2,4-D + 0.5 mg/LBAP + 0.5 mg/LNAA. Following the development of the root system, the resulting clones are prepared for ex vitro transfer. Acclimatization is conducted in a soil substrate under a polyethylene cover with a 16-hour photoperiod to support the transition to ex vitro conditions.

Authors

Svetlana M. Zaytseva^1,3*, Candidate of Biology, Assoc. Prof.; ResearcherID: AAE-5391-2022,
ORCID: https://orcid.org/0000-0001-9137-3774
Elizaveta L. Bolotina¹, Postgraduate Student; ORCID: https://orcid.org/0009-0007-9006-6044
Elena A. Kalashnikova², Doctor of Biology, Prof.; ORCID: https://orcid.org/0000-0002-2655-1789
Rima N. Kirakosyan¹, Candidate of Biology, Assoc. Prof.;
ORCID: https://orcid.org/0000-0002-5244-4311

Affiliation

¹Department of Biotechnology, Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, ul. Timiryazevskaya, 49, Moscow, Russian Federation, 127434; smzaytseva@yandex.ru*, lizavetarodbol@yandex.ru, mia41291@mail.ru
²Department of Сell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology,
Russian Academy of Sciences, ul. Botanicheskaya, 35, Moscow, Russian Federation, 127276; kalash0407@mail.ru
³Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Scriabin,
ul. Akademika Skryabina, 23, Moscow, Russian Federation, 109472; smzaytseva@yandex.ru*

Keywords

Sequoia sempervirens, clones, relict plants, in vitro

For citation

Zaytseva S.M., Bolotina E.L., Kalashnikova E.A., Kirakosyan R.N. Clonal Micropropagation Technology of Relict Gymnosperms Sequoia sempervirens (D. Don) Endl. Lesnoy Zhurnal = Russian Forestry Journal, 2026, no. 2, pp. 73–87. (In Russ.). https://doi.org/10.37482/0536-1036-2026-2-73-87

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