Features of Triploid Aspen Clonal Micropropagation Using Modern Growth-Stimulating Preparations. P. 183–194

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630*232

DOI:

10.37482/0536-1036-2023-2-183-194

Abstract

The creation of fast-growing triploid aspen plantations in the area of wood processing enterprises has high importance in terms of increasing demand for deciduous wood associated with the development of board production and the prospects for the introduction of innovative technologies for deep processing of wood. The article presents the results of studies of triploid aspen clones of Kostroma origin at the stages of “introduction to in vitro culture”, “proper micropropagation”, and “rooting of microshoots” with the use of growth-regulating substances. At the “introduction culture to in vitro” stage, the most effective sterilizing agents were silver nitrate with the concentration of 0.2 %, Lysoformin 3000, 5 % applied for 15 min and sulema, 0.2 % used for 10 min. At the “proper micropropagation” stage, the total length of triploid aspen shoots did not differ significantly depending on the composition of the nutrient media studied but was slightly exceeded the options with Woody Plant Medium. An increase by 1.2–2.6 times was observed in the total length of triploid aspen microshoots in vitro, when the concentration of 6-benzylaminopurine in the nutrient medium was increased from 0.5 to 1.0 mg/L. The maximum value of the total length (5.6 cm) of tryploid aspen microshoots in vitro was observed on Woody Plant Medium at the 6-benzylaminopurine concentration of 1.0 mg/L and the presence of Epin-Extra preparation of 0.5 mg/L. At the “rooting of microshoots” stage the number, average, and total length of triploid aspen roots in vitro had no statistically significant differences depending on the nutrient medium composition. Increasing the concentrations of indole-3-butyric acid and indole-3-acetic acid from 1.0 to 2.0 mg/L in the nutrient medium increased the total length of regenerated plant roots of triploid aspen in vitro by 2.3–2.4 times. The maximum total length (5.1 cm) of triploid aspen roots in vitro was observed on Woody Plant Medium with indole-3-butyric acid at a concentration of 1.0 mg/L. Clonal micropropagation appears to be a perspective for accelerated production of elite planting material of triploid aspen for the purposes of laying out timber plantations.

Authors

Sergey S. Makarov^1,4*, Doctor of Agriculture, Senior Research Scientist; ResearcherID: AAK-9829-2021, ORCID: https://orcid.org/0000-0003-0564-8888
Evgeniy S. Bagaev², Candidate of Agriculture, Leading Engineer; ORCID: https://orcid.org/0000-0001-6527-4748
Anton I. Chudetsky², Leading Engineer; ResearcherID: H-1210-2019, ORCID: https://orcid.org/0000-0003-4804-7759
Irina B. Kuznetsova³, Candidate of Agriculture, Assoc. Prof.; ResearcherID: AAB-4568-2021, ORCID: https://orcid.org/0000-0001-5011-3271
Olga P. Lebedeva⁴, Training Master; ResearcherID: G-7967-2019, ORCID: https://orcid.org/0000-0002-5282-4904
Alexandr M. Antonov⁴, Candidate of Agriculture, Assoc. Prof.; ResearcherID: R-4605-2019, ORCID: https://orcid.org/0000-0002-7076-233X

Affiliation

¹Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, ul. Timiryazevskaya, 49, Moscow, 127434, Russian Federation; makarov_serg44@mail.ru*
²Central European Forest Experimental Station, prosp. Mira, 134, Kostroma, 156013, Russian Federation; ce-los-lh@mail.ru, a.chudetsky@mail.ru
³Kostroma State Agricultural Academy, ul. Uchebnyy gorodok, Karavaevskaya s/a, 34, p. Karavaevo, Kostroma District, Kostroma Region, 156530, Russian Federation; sonnereiser@yandex.ru
⁴Northern (Arctic) Federal University named after M.V. Lomonosov, Naberezhnaya Severnoy Dviny, 17, Arkhangelsk, 163002, Russian Federation; makarov_serg44@mail.ru*, o.lebedeva@narfu.ru, a.antonov@narfu.ru

Keywords

triploid aspen, clone, forest plantations, clonal micropropagation, in vitro, organogenesis, rhizogenesis, growth-regulating substances

For citation

Makarov S.S., Bagaev E.S., Chudetsky A.I., Kuznetsova I.B., Lebedeva O.P., Antonov A.M. Features of Triploid Aspen Clonal Micropropagation Using Modern Growth-Stimulating Preparations. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 2, pp. 183–194. (In Russ.). https://doi.org/10.37482/0536-1036-2023-2-183-194

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