Correlation of Characteristics of the Needle Pigment Combination of Representatives of the Genus Larch (Larix Mill.) in the Arboretum of the Sergachsky Forestry in the Nizhny Novgorod Region

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UDС

582.475.2+581.174.1/.2

DOI:

10.17238/issn0536-1036.2018.3.43

Abstract

Larch as a forest-forming species, occupies vast territories of the forest fund of the Russian Federation, providing a species diversity of dendroflora. Within the range, larch creates highly productive, stable, pure and mixed plantations. Larch distribution is rather limited in the Nizhny Novgorod region. This species is of interest for plantation forestry, urban landscaping, protective forestation. This attracts attention to a multifaceted assessment of the prospects for the further species use of the genus larch. The effective attraction for of these speciesin the newly created artificial plantations is impossible without a study of their adaptive capacity. Green pigments and carotenoids determine the normal operation of the plant photosynthetic apparatus. Their content and ratio in needles correlate with resistance and can be the indica tor of resistance to unfavorable environmental factors. In this regard, the author faces a task of establishing the correlation ratio and the nature of the dependence between characteristics of the needles pigment combination of various species of larch during the introduction in the Nizhny Novgorod region. The study subject is autochthonous and introduced species of larch; their representatives are located in the collection plantings of the dendrological complex “Yavleika” in the Sergachsky forestry of the Nizhny Novgorod region. The total number of accounting trees is 30 (10 trees of each type). Species composition is represented by Siberian larch (Larix sibirica Ledeb.), Dahurian (Larix gmelinii (Rupr.) Kuzen.) and Sukachev’s larch (Larix sukaczewii Djil. Dyl). 90 samples are prepared for the analysis. Spectrophotometric analysis is applied as the most accurate for the quantitative estimation of pigments of the leaf apparatus. The concentration of pigments is calculated by the equations of Wetstein and Holm. The result of the analysis is the correlation matrices and the equations of interdependence of values of main characteristics of the pigment combination of larch needles. The linkage between the studied characteristics for all the larch species are determined. Experimental values of the Student's test turn out to be higher than the tabulated ones; the accuracy factor of the experiment does not exceed the values permissible for biological studies of the five-percent level. The numerical values of the content of all the main pigments indicate a correlation.

Authors

A.O. Esichev, Postgraduate Student

Affiliation

Nizhny Novgorod State Agricultural Academy, pr. Gagarina, 97, Nizhny Novgorod, 603107, Russian Federation; e-mail: andrey.esichev@mail.ru

Keywords

larch, needles, chlorophyll-a, chlorophyll-b, carotenoid, introduction, interspecies variation, correlation, regression

For citation

Esichev A.O. Correlation of Characteristics of the Needle Pigment Combination of Representatives of the Genus Larch (Larix Mill.) in the Arboretum of the Sergachsky Forestry in the Nizhny Novgorod Region. Lesnoy zhurnal [Forestry journal], 2018, no. 3, pp. 43–53. DOI: 10.17238/issn0536-1036.2018.3.43

References

1. Besschetnov V.P. Perspektivy razvitiya plantatsionnogo lesovodstva na selektsionno-geneticheskoy osnove v Nizhegorodskoy oblasti [Prospects for the Development of Plantation Forestry on the Breeding and Genetic Basis in the Nizhny Novgorod Region]. Lesnoy kompleks Nizhegorodskogo Povolzh'ya: problemy, sostoyanie i perspektivy razvitiya [Forest Complex of the Nizhny Novgorod Volga Region: Problems, State and Development Prospects]. Nizhny Novgorod, NNSAA Publ., 2006, pp. 21–32. (In Russ.)
2. Besschetnov V.P., Besschetnova N.N., Esichev A.O. Otsenka fiziologicheskogo sostoyaniya predstaviteley roda listvennitsa (Larix Mill.) v usloviyakh Nizhegorodskoy oblasti [Physiological State Evaluation of Representatives of the Genus Larch (Larix Mill.) in the Nizhny Novgorod Region]. Lesnoy zhurnal [Forestry journal], 2018, no. 1, pp. 9–17. 3. Besschetnov V.P., Chishkina A.V., Rubtsov B.V. Sravnitel'naya otsenka taksatsionnykh pokazateley eli evropeyskoy v lesnykh kul'turakh i promyshlennykh plantatsiyakh na severe Nizhegorodskoy oblasti [Comparative Assessment of Taxation Indicators of Norway Spruce in Forest Cultures and Industrial Plantations in the North of the Nizhny Novgorod Region]. Lesnoy kompleks Nizhegorodskogo Povolzh'ya: problemy, sostoyanie i perspektivy razvitiya [Forest Complex of the Nizhniy Novgorod Region: Problems, State and Development Prospects]. Nizhniy Novgorod, NNSAA Publ., 2006, pp. 32–36. (In Russ.)
4. Bokova V.V. Perspektivy sozdaniya plantatsionnykh kul'tur eli v Nizhegorodskoy oblasti [Prospects for the Creation of Plantation Spruce Crops in the Nizhny Novgorod Region]. Zashchitnoe lesorazvedenie, melioratsiya zemel' i problemy zemledeliya v Rossiyskoy Federatsii: materialy mezhdunar. nauch.-prakt. konf., g. Volgograd, 23–26 sent. 2008 g. [Protective Afforestation, Land Reclamation and Farming Problems in the Russian Federation: Proc. Intern. Sci. Practical Conf., Volgograd, September 23–26, 2008]. Volgograd, All-Russ. Agroforestry Research Institute Publ., 2008, pp. 138–140. (In Russ.)
5. Bokova V.V. Sostoyanie i perspektivy razvitiya plantatsionnykh kul'tur v Nizhegorodskoy oblasti i Rossii [State and Prospects of Development of Plantation Crops in the Nizhny Novgorod Region and Russia]. Vestnik Nizhegorodskoy gosudarstvennoy sel'skokhozyaystvennoy akademii [Vestnik of Nizhny Novgorod State Agricultural Academy], 2012, vol. 1, pp. 284–290.
6. Gosudarstvennaya programma Rossiyskoy Federatsii «Razvitie lesnogo khozyaystva na 2013–2020 gg.»: utv. rasporyazheniem Pravitel'stva Rossiyskoy Federatsii ot 28 dek. 2012 g. № 2593-r [The State Program of the Russian Federation “Forestry Development for 2013‒2020”: Approved by the Decree of the Government of the RF of 28 December 2012, No. 2593-r]. Sobranie zakonodatel'stva RF [Collected Acts of the Russian Federation], 2013, no. 2. 230 p.
7. Demakov Yu.P., Krasnov V.G., Isaev A.V. Zakonomernosti stroeniya i razvitiya drevostoya na plantatsii listvennitsy sibirskoy v kratkopoymennom ekotope [Structural and Developmental Patterns of the Stand in the Siberian Larch Plantations in the Bottomland Ecotope]. Vestnik Povolzhskogo gosudarstvennogo tekhnologicheskogo universiteta. Ser.: Les. Ekologiya. Prirodopol'zovanie [Vestnik of Volga State University of Technology. Ser.: Forest. Ecology. Nature Management], 2017, no. 1(33), pp. 36–49.
8. Dospekhov B.A. Metodika polevogo opyta (s osnovami statisticheskoy obrabotki rezul'tatov issledovaniy) [Field Test Methodology (with the Basics of Statistical Processing of Research Results)]. Moscow, Agropromizdat Publ., 1985. 351 p. (In Russ.)
9. Esichev A.O. Sravnitel'naya otsenka assortimenta klonov plyusovykh derev'ev listvennitsy Sukacheva (L. Sukaczewii Djil) na lesosemennoy plantatsii v Nizhegorodskoy oblasti [Comparative Assessment of Plus Trees Clones of Sukachev Larch (L. Sukaczewi Djil) Grown on the Forest-Seed Plantation in Nizhegorodsky Region]. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta [Izvestia of Orenburg State Agrarian University], 2016, no. 3(59), pp. 66–68.
10. Zarubina L.V., Konovalov V.N. Osobennosti sezonnoy dinamiki pigmentov v list'yakh rasteniy sosnyaka kustarnichkovo-sfagnovogo [Seasonal Dynamics’ Peculiarities of Leaves’ Pigments in Fruticulose-sphagnous Pine Stands]. Lesnoy zhurnal [Forestry journal], 2009, no. 4, pp. 24–32.
11. Koptev V.V., Bogomyagkikh V.A., Trifonova M.F. Osnovy nauchnykh issledovaniy i patentovedeniya [Fundamentals of Scientific Research and Patent Science]. Moscow, Kolos Publ., 1993. 144 p. (In Russ.)
12. Lakin G.F. Biometriya [Biometrics]. Moscow, Vysshaya shkola Publ., 1980. 293 p. (In Russ.)
13. Prikaz Ministerstva prirodnykh resursov i ekologii Rossiyskoy Federatsii ot 18 avg. 2014 g. № 367 «Ob utverzhdenii Perechnya lesorastitel'nykh zon Rossiyskoy Federatsii i Perechnya lesnykh rayonov Rossiyskoy Federatsii» (s izmeneniyami na 21 marta 2016 g.): zaregistrirovano v Ministerstve yustitsii RF 29 sent. 2014 g., № 34186 [Order of the Ministry of Natural Resources and Ecology of the Russian Federation on August 18, 2014, No. 367 “On Approval of the List of Forest Plant Zones of the Russian Federation and the List of Forest Regions of the Russian Federation” (as Amended on March 21, 2016): Registered in the Ministry of Justice of the Russian Federation on 29 September 2014, No. 34186]. Available at: http://docs.cntd.ru/document/420224339 (accessed 30.03.2018).
14. Prikaz Federal'nogo Agentstva lesnogo khozyaystva ot 28 marta 2016 g. № 100 «O vnesenii izmeneniy v prikaz Rosleskhoza ot 08.10.2015 № 353 «Ob ustanovlenii lesosemennogo rayonirovaniya» [Order of the Federal Forestry Agency No. 100 of March 28, 2016 “On Amendments to the Order of the Federal Forestry Agency No. 353 of 08.10.2015 “On the Establishment of Forest Seed Zoning”]. Sobranie zakonodatel'stva RF [Collected Acts of the Russian Federation], 2013, no. 2. 230 p.
15. Prozherina N.A., Nakvasina E.N. Vnutri- i mezhvidovye metabolicheskie osobennosti u listvennitsy pri adaptatsii na severe [Intra- and Interspecies Metabolic Peculiarities of Larch under Adaptation to North]. Lesnoy zhurnal [Forestry journal], 2008, no. 5, pp. 30–36. 
16. Khodasevich E.V. Fotosinteticheskiy apparat khvoynykh: ontogeneticheskiy aspekt [Photosynthetic Apparatus of Conifers: Ontogenetic Aspect]. Minsk, Nauka i tekhnika Publ., 1982. 199 p. (In Russ.)
17. Shlyk A.A. Opredelenie khlorofillov i karotinoidov v ekstraktakh zelenykh list'ev [Determination of Chlorophylls and Carotenoids in Extracts of Green Leaves]. Biokhimicheskie metody v fiziologii rasteniy [Biochemical Methods in Plant Physiology]. Moscow, Nauka Publ., 1971, pp. 154–170. (In Russ.)
18. Duysens L.N.M., Amesz J., Kamp B.M. Two Photochemical Systems in Photosynthesis. Nature, 1961, vol. 190, no. 4775, pp. 510–511.
19. Lichtenthaler H.K., Buschmann C. Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy. Current Protocols in Food Analytical Chemistry. Ed. by R.E. Wrolstad, T.E. Acree, H. An, E.A. Decker, M.H. Penner, D.S. Reid, S.J. Schwartz, C.F. Shoemaker, P. Sporns. New York, John Wiley and Sons, 2001, F4.3.1-F4.3.8. Available at: http://www.thyssen-web.de/assets/files/fd_documents/sp_buche/uv_vis_pigmente.pdf (accessed 10.05.2014).
20. Miazek K., Ledakowicz S. Chlorophyll Extraction from Leaves, Needles and Microalgae: a Kinetic Approach. International Journal of Agricultural and Biological Engineering, 2013, vol. 6, no. 2, pp. 107–115. Available at: http://www.ijabe.org/index.php/ ijabe/ article/ viewFile/ 642/580 (accessed 17.07.2014).
21. Niinemets Ü. Stomatal Conductance Alone does not Explain the Decline in Foliar Photosynthetic Rates with Increasing Tree Age and Size in Picea abies and Pinus sylvestris. Tree Physiology, 2002, vol. 22, iss. 8, pp. 515–535. Available at: http://treephys.oxfordjournals.org/content/22/8/515.full.pdf+html (accessed 27.03.2012).
22. Niinemets Ü., Ellsworth D.S., Lukjanova A., Tobias M. Site Fertility and the Morphological and Photosynthetic Acclimation of Pinus sylvestris Needles to Light. Tree Physiology, 2001, vol. 21, iss. 17, pp. 1231–1244. Available at: http://treephys.oxfordjournals. org/content/21/17/1231.full.pdf+html (accessed 27.03.2012).
23. Niinemets Ü., Ellsworth D.S., Lukjanova A., Tobias M. Dependence of Needle Architecture and Chemical Composition on Canopy Light Availability in Three North American Pinus Species with Contrasting Needle Length. Tree Physiology, 2002, vol. 22, iss. 11, pp. 747–761. Available at: http://treephys.oxfordjournals.org/content/22/11/747.full.pdf+html (accessed 29.03.2012).
24. Wellburn A.R. The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. Journal of Plant Physiology, 1994, vol. 144, iss. 3, pp. 307–313. Available at: http://www.thyssen-web.de/assets/files/fd_documents/sp_buche/Wellburn.pdf (accessed 29.03.2012).

Received on March 06, 2018