Extraction of Biologically Active Substances from the Bark and Leaves of Trees of the Salicaceae Family. С. 173-184

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UDС

678.031:54-112

DOI:

10.37482/0536-1036-2025-4-173-184

Abstract

The process of extraction of biologically active substances from non-woody parts of trees (bark and leaves) of willow and aspen is described in the article. The instrumentation for extracting salicin and quercetin from the bark and leaves of trees of the Salicaceae family is presented. A method for experimental research and obtaining of biologically active substances is presented. It has been found that an increase in the extractant consumption from 20 to 100 l/min at the same temperature (75 °С) and 95 % impregnation leads to a 2-fold acceleration of the process. Local temperature changes across the layers of the material have been described. A slight lag in heating of subsequent layers of the material by 1–2 min has been determined, which is due to the thermal inertia of the material and the peculiarities of heat transfer within the layer. It takes 7 min to achieve full heating. A kinetic curve of the concentration of extracted biologically active substances in the extract has been constructed. The resulting dependencies and comparative data allow us to recommend rational modes of extraction of biologically active substances from the bark and leaves of willow and aspen trees. According to the results of the research, the optimal temperature for quercetin extraction has been recommended to be 50 °С. It has also been found that increasing the temperature to 75 °С leads to a noticeable increase in the yield of salicin. Moreover, it has been revealed that the largest amount of quercetin is found in aspen (1.4 %), with 11.4 % more quercetin in the leaves than in the bark; the main amount of salicin is found in the bark of both species, but in willow bark there is an excess of 35 %; the rational solvent concentration is 60 %; the duration of extraction is 40–45 min.

Authors

Rushan G. Safin, Doctor of Engineering, Prof.; ResearcherID: Q-8575-2017,
ORCID: https://orcid.org/0000-0002-5790-4532
Kirill V. Valeev*, Candidate of Engineering, Assoc. Prof.;
ResearcherID: HPC-5151-2023, ORCID: https://orcid.org/0000-0002-5537-9332
Dilara F. Ziatdinova, Doctor of Engineering, Prof.; ResearcherID: U-1572-2017,
ORCID: https://orcid.org/0000-0003-2801-4642

Affiliation

Kazan National Research Technological University, ul. K. Marksa, 68, Kazan, 420015, Russian Federation; safin@kstu.ru, kirval116@mail.ru*, Ziatdinova2804@gmail.com

Keywords

Salicaceae, extraction, processing, biologically active substances, salicin, quercetin, leaves, bark

For citation

Safin R.G., Valeev K.V., Ziatdinova D.F. Extraction of Biologically Active Substances from the Bark and Leaves of Trees of the Salicaceae Family. Lesnoy Zhurnal = Russian Forestry Journal, 2025, no. 4, pp. 173–184. (In Russ.). https://doi.org/10.37482/0536-1036-2025-4-173-184

References

1. Grishkova L.A., Demchenko E.A., Kovalen V.E., Land V.Ya., Markichev I.I., Nekrasova V.B. Method of Processing Aspen Bark: Author’s Certificate USSR 986380. (In Russ.).
2. Blazhej A., Shutyj L. Phenolic Compounds of Plant Origin. Moscow, Mir Publ., 1977. 240 p. (In Russ.).
3. Dement’eva T.M. Study of the Bark and Shoots of the Babylonian Willow (Salix babylonica L.) and the Hybrid of the Babylonian Willow and the White Willow (Salix babylonica L. × Salix alba L.), Growing in the North Caucasus: Cand. Diss. Pharm. Sci. Diss., 2016. 154 p. (In Russ.).
4. Demchenkova E.Yu. Standardization of Medicinal Plant Raw Materials and Phytopreparations for Antioxidant Content Using the Amperometric Method: Cand. Pharm. Sci. Diss. Abs. Moscow, 2012. 25 p. (In Russ.).
5. Zhmatova G.V., Nefedov A.N., Gordeev A.S., Kilimnik A.B. Intensification Methods of Extracting Biologically Active Substances from Vegetative Raw Materials. Vestnik TGTU = Transactions TSTU, 2005, vol. 11, no. 3, pp. 701–707. (In Russ.).
6. Litvinenko V.I. Chemistry of Natural Flavonoids and Creation of Preparations During Complex Processing of Plant Raw Materials: Doc. Chem. Sci. Diss. Abs. Kharkiv, Kharkiv Research Chemical-Pharmaceutical Institute, 1990. 29 p. (In Russ.).
7. Lomako E.V., Kuzmichova N.A. Application of Surfactants in the Analysis of Medicinal Vegetative Raw Material Containing Flavonoids. Vestnik farmatsii, 2014, no. 3 (65), pp. 42–49. (In Russ.).
8. Maslennikova K.A., Konyukhova O.M., Kanarskii A.V. Phenolglycosides of Plants of the Salicaceae Family. Vestnik Kazanskogo tekhnologicheskogo universtiteta = Herald of Technological University, 2014, vol. 17, no. 14, pp. 383–386. (In Russ.).
9. Matveenko E.V., Velichko N.A., Ushanov S.V., Ayoshina E.N. Mathematical Model of the Extraction Process of Juniperus sibirica burgsd Wood Greenery with an Aqueous Solution of Ethyl Alcohol. Problems of Modern Agricultural Science: Proceedings of the International Correspondence Scientific Conference. Krasnoyarsk, 2015, pp. 169–174. (In Russ.).
10. Poyarkova N.M., Saparklycheva S.E. The Physiological Role of Phenolic Compounds. Agrarnoe obrazovanie i nauka = Agrarian Education and Science, 2019, no. 4. (In Russ.).
11. Rassypnova S.S., Turetskova V.F., Zverev Ya.F. Study of the Anti-Inflammatory Effect of Populus tremula (Salicaceae) Bark Extract and its Constituent Phenolic Compounds. Rastitelnye Resursy, 2010, vol. 46, no. 3, pp. 103–108. (In Russ.).
12. Safin R.G., Prosvirnikov D.B., Arslanova G.R., Valeev K.V., Ziatdinova D.F., Guryanov D.A. Mathematical Description of the Extraction Process of Phenolic Compounds. Derevoobrabativaushaya promishlennost’ = Woodworking Industry, 2022, no. 1, pp. 62–70. (In Russ.).
13. Ushanova V.M., Lebedeva O.I., Devyatlovskaya A.N. Fundamentals of Scientific Research. Part 2. Quality Control and Extraction of Plant Raw Materials. Krasnoyarsk, Siberian State Technological University Publ., 2004. 168 p. (In Russ.).
14. Shvets P., Khalabala M. Acetylsalicylic Acid – a Medicine Tested by Generations (on the Centenary of Acetylsalicylic Acid). Slovakofarma revu. Kyiv, 2002, pp. 66–68. (In Russ.).
15. Shegel’man I.R., Kuznetsov A.V. Efficient Use of Forest Resources. Petrozavodsk, Petrozavodsk State University, 2008. 88 p. (In Russ.).
16. Jokić S., Jerković I., Rajić M., Aladić K., Bilić M., Vidović S. SC-CO2 Extraction of Vitex agnus-castus L. Fruits: The Influence of Pressure, Temperature and Water Presoaking on the Yield and GC–MS Profiles of the Extracts in Comparison to the Essential Oil Composition. The Journal of Supercritical Fluids, 2017, vol. 123, pp. 50–57. https://doi.org/10.1016/j.supflu.2016.12.007
17. Safina A.V., Valeev K.V., Ziatdinova D.F. Method for Determining the Mass Conductivity Coefficient of Wood when Extracting Biologically Active Substances from it. Journal of Physics: Conference Series, 2022, vol. 2373, art. no. 042004. https://doi.org/10.1088/1742-6596/2373/4/042004
18. Safin R.G., Prosvirnikov D.B., Arslanova G.R. Parametric Multidimensional Modeling of Extraction Processes in the Wood Chemical, Food and Pharmaceutical Industries. Proceedings of the 7th International Conference on Industrial Engineering (ICIE 2021). Springer, Cham, 2021, pp. 286–297. https://doi.org/10.1007/978-3-030-85230-6_34
19. Soares J.F., Zabot G.L., Tres M.V., Lunelli F.C., Rodrigues V.M., Friedrichc M.T., Pazinatto C.A., Bilibio D., Mazutti M.A., Carniel N., Priamo W.L. Supercritical CO2 Extraction of Black Poplar (Populus nigra L.) Extract: Experimental Data and Fitting of Kinetic Parameters. The Journal of Supercritical Fluids, 2016, vol. 117, pp. 270–278. https://doi.org/10.1016/j.supflu.2016.07.005