Variability of the Photosynthetic Pigment Complex in Spruce Trees with Different Seed Scale Shapes in the Northern Taiga of the Arkhangelsk Region. P. 96–109

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UDС

582.475.2:631.524.6

DOI:

10.37482/0536-1036-2026-4-96-109

Abstract

Terrain of the Arkhangelsk region is part of the introgressive hybridization zone of Siberian spruce (Picea obovata Ledeb.) and European spruce (Picea abies (L.) Karst.) on the East European Plain. In many species of the Pinaceae family, the shape of the seed scales contains a wealth of genetic information specific to both the species and its varieties. The research aimed at determining the seasonal dynamics of the photosynthetic pigment complex in Siberian spruce and hybrid spruce with a predominance of Siberian spruce traits in the northern taiga of the Arkhangelsk Region. The field studies were carried out in blueberry spruce forests on low-podzolic loamy soils overlying morainic loams, typical in the northern taiga near the mouth of the Northern Dvina River (64°30ʹ N, 41°00ʹ E). We have labelled 10 Siberian spruce trees and 10 hybrid spruce trees with a predominance of Siberian spruce traits, determined by the seed scale shape at the permanent trial plots. Samples of annual (shadow) needles were harvested from the lower part of the crown at various times of the year in 2023. Spectroscopic methods allowed us to determine the chlorophyll and carotenoid content in pine needles and to calculate the proportion of chlorophylls in the light-harvesting complex. The results revealed similarities in the dynamics of relative parameters of the photosynthetic pigment complex in annual needles of Siberian spruce and hybrid spruce with a predominance of Siberian spruce traits. Meanwhile, the research shows that during the summer and fall seasons, right up until the end of the growing season and as they prepare for winter dormancy, the spruce trees studied accumulate green pigments, with the hybrid form exhibiting this process more actively. This increases the protective role of carotenoids. The range of variation in the proportion of chlorophylls in the light-harvesting complex of Siberian spruce and hybrid spruce with a predominance of Siberian spruce traits from May to November averages 55–62 % per month, which is due to the adaptation of their photosynthetic apparatus to the ecological conditions of high latitudes.

Funding: The research was carried out as part of the state assignment of the N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences titled “Study of the Forest Ecosystems Resilience in Subarctic Regions of the Russian European North” (Project FUUW-2025-0003, Registration No. 125021902596-8).

Authors

Sergei N. Tarkhanov*, Doctor of Biology, Chief of Laboratory, Chief Research Scientist; ResearcherID: ABG-7237-2020, ORCID: https://orcid.org/0000-0001-9037-8995
Ekaterina A. Pinaevskaya, Candidate of Biology, Senior Research Scientist; ResearcherID: ABB-6293-2020, ORCID: https://orcid.org/0000-0003-1877-1412
Yuliya E. Aganina, Candidate of Biology, Research Scientist; ResearcherID: ABB-6305-2020, ORCID: https://orcid.org/0000-0002-6069-8979
Aleksandr S. Pakhov, Junior Research Scientist; ResearcherID: HRB-3337-2023, ORCID: https://orcid.org/0000-0002-2362-8840

Affiliation

N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences, Arkhangelsk, Russia; tarkse@yandex.ru*, aviatorov8@mail.ru, julja-a30@rambler.ru, aleksander.pakhoff@yandex.ru

Keywords

spruce, seed scale shape, Siberian spruce, Picea obovata Ledeb., hybrid spruce with a predominance of Siberian spruce traits, chlorophylls a and b, carotenoids, Arkhangelsk region, northern taiga, photosynthetic pigment complex, seasonal dynamics of pigme

For citation

Tarkhanov S.N., Pinaevskaya E.A., Aganina Yu.E., Pakhov A.S. Variability of the Photosynthetic Pigment Complex in Spruce Trees with Different Seed Scale Shapes in the Northern Taiga of the Arkhangelsk Region. Lesnoy Zhurnal = Russian Forestry Journal, 2026, no. 4, pp. 96–109. (In Russ.). https://doi.org/10.37482/0536-1036-2026-4-96-109

References

1. Besschetnova N.N., Besschetnov V.P., Ershov P.V. Genotypic Conditionality of Plus Tree Needle Pigment Composition of Norway Spruce. Lesnoy Zhurnal = Russian Forestry Journal, 2019, no. 1, pp. 63–76. (In Russ.). https://doi.org/10.17238/issn0536-1036.2019.1.63

2. Esichev A.O. Correlation of Characteristics of the Needle Pigment Combination of Representatives of the Genus Larch (Larix Mill.) in the Arboretum of the Sergachsky Forestry in the Nizhny Novgorod Region. Lesnoy Zhurnal = Russian Forestry Journal, 2018, no. 3, pp. 43–53. (In Russ.). https://doi.org/10.17238/issn0536-1036.2018.3.43

3. Ivanov L.A., Ronzhina D.A., Yudina P.K., Kalashnikova I.V., Ivanova L.A., Zolotareva N.V. Seasonal Dynamics of the Chlorophyll and Carotenoid Content in the Leaves of Steppe and Forest Plants on Species and Community Level. Fiziologiya rastenii = Russian Journal of Plant Physiology, 2020, vol. 67, no. 3, pp. 278–288. (In Russ.). https://doi.org/10.31857/S0015330320030112

4. Virgin Spruce Forest of the North: Biodiversity, Structure, Functions. Ed. By K.S. Bobkova, E.P. Galenko. Saint Petersburg, Nauka Publ., 2006. 337 p. (In Russ.).

5. Koropachinskiy I.Y., Potemkin O.N., Rudikovskiy A.V., Kuznetsova E.V. Polymorphism and Structure of Populations of Siberian Spruce (Picea obovata Ledeb.) at the Northern Limits of the Species Distribution. Sibirskij Ekologicheskij Zhurnal = Contemporary Problems of Ecology, 2012, vol. 19, no. 2, pp. 175–184. (In Russ.).

6. Maslova T.G., Mamushina N.S., Sherstneva O.A., Bubolo L.S., Zubkova E.K. Structural and Functional Changes in the Photosynthetic Apparatus of Winter-Vegetating Coniferous Plants in Different Seasons. Fiziologiya rastenij = Russian Journal of Plant Physiology, 2009, vol. 56, no. 5, pp. 672–681. (In Russ.).

7. Petrov K.A., Sofronova V.E., Bubyakina V.V., Perk A.A., Tatarinova T.D., Ponomarev A.G., Chepalov V.A., Okhlopkova Zh.M., Vasilieva I.V., Maksimov T.Kh. Woody Plants of Yakutia and Low-Temperature Stress. Fiziologiya rastenij = Russian Journal of Plant Physi¬ology, 2011, vol. 58, pp. 1011–1019. (In Russ.). https://doi.org/10.1134/S1021443711060148

8. Popov P.P. European and Siberian Spruce: Structure, Intergradation and Differentiation of Population Systems. Novosibirsk, Nauka Publ., 2005. 230 p. (In Russ.).

9. Popov P.P. Form Structure and Taxonomic Affiliation of Intermediate Populations of European and Siberian Spruce. Lesovedenie = Russian Journal of Forest Science, 2007, no. 4, pp. 59–67. (In Russ.).

10. Popov P.P. The Phenotypic Diversity of Cone Scales in the Northern Dvina Spruce Populations, and Their Taxonomic Interpretation. Botanicheskij zhurnal, 2010, vol. 95, no. 5, pp. 647–656. (In Russ.).

11. Popov P.P. The Distribution of Individuals of Intermediate Form in the Populations of Norway and Siberian Spruces. Sibirskij lesnoj zhurnal = Siberian Journal of Forest Science, 2018, no. 4, pp. 13–19. (In Russ.). https://doi.org/10.15372/SJFS20180402

12. Popov P.P., Kazantseva M.N., Arefyev S.P. Phenotypic Structure of Spruce Populations in the European North of Russia. Lesnoy Zhurnal = Russian Forestry Journal, 2021, no. 2, pp. 9–20. (In Russ.). https://doi.org/10.37482/0536-1036-2021-2-9-20

13. Pravdin L.F. European Spruce and Siberian Spruce in the USSR. Moscow, Nauka, Publ., 1975. 178 p. (In Russ.).

14. Workshop on Plant Physiology. Ed. by N.N. Tretyakov, T.V. Karnaukhova, L.A. Panichkin, N.V. Pilshchikova, V.G. Zemskiy, L.V. Mozhaev, A.E. Petrov-Spiridonov, Ya.M. Gellerman, M.N. Kondratyev, M.I. Kalinkievich, E.E. Krastina, K.I. Kamenskaya. Moscow, Agropromizdat Publ., 1990. 271 p. (In Russ.).

15. Pridacha V.B., Tumanik N.V., Semin D.E. Abiotic Impacts оn Nitrogen Content, Pigment Complex and Water Exchange Parameters of Pinus sylvestris (Pinaceae) Needles on Post-Industrial Lands. Rastitel’nyye resursy = Vegetation Resources, 2024, vol. 60, no. 3, pp. 63–79. (In Russ.). https://doi.org/10.31857/S0033994624030042

16. Rozentsvet O.A., Bogdanova E.S., Nesterov V.N., Shuyskaya E.V., Ivanova L.A. Structure of Chlorenchyma and the Profile of Membrane Lipids in Leaves of Halophytic Chenopodiaceae Species with Different Types of Photosynthesis. Fiziologiya rastenii = Russian Journal of Plant Physiology, 2022, vol. 69, no. 1, pp. 68–79. (In Russ.). https://doi.org/10.31857/S0015330322010171

17. Sklyarov G.A., Sharova A.S. Soils of Forests of the European North. Moscow, Nauka Publ., 1970. 271 p. (In Russ.).

18. Sofronova V.E., Chepalov V.A., Dymova O.V., Golovko T.K. The Role of Pigment System of an Evergreen Dwarf Shrub Ephedra Monosperma in Adaptation to the Climate of Central Yakutia. Fiziologiya rastenii = Russian Journal of Plant Physiology, 2014, vol. 61, no. 2, pp. 266–274. (In Russ.). https://doi.org/10.7868/S001533031401014X

19. Tarkhanov S.N. Population Variability of Finnish Spruce from the Seed Scale Forms in Northern Arkhangelsk Oblast. Lesovedenie = Russian Journal of Forest Science, 2019, no. 3, pp. 208–214. (In Russ.). https://doi.org/10.1134/S0024114819020116

20. Titova M.S. Content of Photosynthetic Pigments in Needles of Picea abies and Picea koraiensis. Vestnik of the Orenburg State University, 2010, no. 12(118), pp. 9–12. (In Russ.).

21. Tishkina E.A., Semkina L.A., Grigoriev A.A. Ecological Plasticity of Juniperus communis L. Needles Pigment Complex in Highland and Lowland Coenopopulations. Lesovedenie = Russian Journal of Forest Science, 2021, vol. 4, no. 4, pp. 354–362. (In Russ.). https://doi.org/10.31857/S0024114821040100

22. Tuzhilkina V.V. Photosynthetic Pigments of Siberian Spruce Needles in the Middle Taiga Forests of the European North-East of Russia. Sibirskij lesnoj zhurnal = Siberian Journal of Forest Science, 2017, no. 1, pp. 65–73. (In Russ.). https://doi.org/10.15372/SJFS20170107

23. Yatsko Ya.N., Dymova O.V., Golovko T.K. Pigment Complex of Ever- And Wintergreen Plants in the Middle Taiga Subzone of the European North-East. Botanicheskii zhurnal = Botanical Journal, 2009, vol. 94, no. 12, pp. 1812–1820. (In Russ.).

24. Aguil S., Ahmad S.H., Reshi Z.Ah., Mahmooduzzafar N., Iqbal M. Physiological and Biochemical Response of Albizza lebbek (L.) Benth. to Coal Smoke Pollution. Pollution Research, 2003, vol. 22, no. 4, pp. 489–493.

25. Dall’Osto L., Lico C., Alric J., Giuliano G., Havaux M., Bassi R. Lutein is Needed for Efficient Chlorophyll Triplet Quenching in the Major LHCII Antenna Complex of Higher Plants and Effective Photoprotection in vivo under Strong Light. BMC Plant Biology, 2006, vol. 6, art. 32. https://doi.org/10.1186/1471-2229-6-32

26. Demmig-Adams B., Adams W.W., III. Photoprotection in an Ecological Context: The Remarkable Complexity of Thermal Energy Dissipation. New Phytologist, 2006, vol. 172, iss. 1, pp. 11–21. https://doi.org/10.1111/j.1469-8137.2006.01835.x

27. Johnson M.P., Havaux M., Triantaphylides Ch., Ksas B., Pascal A.A., Robert B., et al. Elevated Zeaxanthin Bound to Oligomeric LHCII Enhances the Resistance of Arabidopsis to Photooxidative Stress by a Lipid Protective, Antioxidant Mechanism. Journal of Biological Chemistry, 2007, vol. 282, iss. 31, pp. 22605–22618. https://doi.org/10.1074/jbc.M702831200

28. Oquist G., Huner N.P.A. Photosynthesis of Overwintering Evergreen Plants. An¬nual Review of Plant Biology, 2003, vol. 54, pp. 329–355. https://doi.org/10.1146/annurev.arplant.54.072402.115741

29. Popov P.P. Form Structure and Geographic Differentiation of Spruce Populations in Northwestern Russia. Russian Journal of Ecology, 2010, vol. 41, no. 5, pp. 378–385. https://doi.org/10.1134/S1067413610050036

30. Sofronova V.E., Antal T.K., Dymova O.V., Golovko T.K. Photoprotective Mechanisms in Photosystem II of Ephedra monosperma During Development of Frost Tolerance. Russian Journal of Plant Physiology, 2014, vol. 61, no. 6, pp. 751–759. https://doi.org/10.1134/S1021443714060181

31. Sofronova V.E., Dymova O.V., Golovko T.K., Chepalov V.A., Petrov K.A. Adaptive Changes in Pigment Complex of Pinus sylvestris Needles upon Cold Acclimation. Russian Journal of Plant Physiology, 2016, vol. 63, no. 4, pp. 433–442. https://doi.org/10.1134/S1021443716040142

32. Verhoeven A. Sustained Energy Dissipation in Winter Evergreens. New Phytologist, 2014, vol. 201, iss. 1, pp. 57–65. https://doi.org/10.1111/nph.12466