Seasonal Dynamics of Content of Antioxidant System Component in Needles of Scots Pine (Pinus sylvestris L.) Trees Situated in the Local Warming Impact Zone. P. 38–57

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581.1:58.02

DOI:

10.37482/0536-1036-2023-2-38-57

Abstract

The seasonal changes in a period of low temperatures and characteristic correlations of some antioxidant system (AOS) components in pine needles influenced by a gas flare are investigated in this study. The parameters taken are total water content (TWC) in needles, peroxidase activity (AP) and selected elements of the antioxidant system of needles, such as content of carotenoids (Car), ascorbic acid (AA), catechins (Cat) and flavanols (Fl). The needles come from Scots pine trees (Pinus sylvestris L.), which grow in a gradient of environmental conditions formed in the zone of thermal field of the gas flare impact (at various distances). The gas flare is situated on the territory of the Khanty-Mansiysk Autonomous Okrug (UGRA) of Russia. Three hypotheses were subject to verification: 1) the thermal field of the gas flare during the low temperature period affects the water exchange and the AOS state of needles; 2) increase in temperature of the environment in the flare impact zone during the autumn-winter period causes the decrease in amount of moisture in needles, which is a trait of oxidative stress in cells; 3) in the functioning of а needle’s AOS, there are correlations between its components, which values depend on distance from the gas flare and environment created by it. It was found that in the thermal field gradient of the flare, there is neither an additional reduction in TWC in needles compared to the background nor signs of change in the state of AOS corresponding to the oxidative stress in the cells. The greatest impact of the gas flare on characteristics of the physiological state of pine needles is observed in the section closest to the flare. It reveals in the higher values of AP, TWC, Car and decrease in concentration of Fl and AA. The seasonal dynamics of the studied traits values do not correlate with the temperature of the environment. It indicates the indirect effect on the regulation of needle’s AOS activity. The factor and correlation analysis of the data indicate a difference in physiological state of pine needles at different distances from the flare. There is no domination of any processes in the functioning of AOS components. The observed correlations between the studied properties change according to distance to the flare. The most stable is a negative AP relationship with ofter parameters, which rise with the increased distance from the flare. The consistency degree of separate AOS components functioning is maximal in background conditions and decreases when approaching the flare. This fact is explained by the modifying effect of the flare on physiological and biochemical processes of the needle’s adaptation to specific climatic conditions of the environment.

Authors

Sergey A. Shavnin¹, Doctor of Biology, Prof., Leading Research Scientist; ResearcherID: L-3389-2018, ORCID: https://orcid.org/0000-0001-6908-3324
Irek A. Yusupov¹, Candidate of Agriculture, Research Scientist; ResearcherID: AAK-4578-2021, ORCID: https://orcid.org/0000-0001-5782-9483
Andrey A. Montile^1*, Junior Research Scientist; ResearcherID: G-4617-2019, ORCID: https://orcid.org/0000-0003-3983-8030
Dmitry Yu. Golikov¹, Candidate of Agriculture, Research Scientist; ResearcherID: D-2177-2016, ORCID: https://orcid.org/0000-0002-8871-1135
Nataliya V. Marina², Candidate of Chemistry, Assoc. Prof.; ResearcherID: AAL-1754-2021, ORCID: https://orcid.org/0000-0002-2641-2981

Affiliation

¹Institute Botanic Garden UBRAS, Ural Branch of the Russian Academy of Sciences, ul. 8 Marta, 202 а, Yekaterinburg, 620144, Russian Federation; sash@botgard.uran.ru, usiaz@mail.ru, org17@mail.ru*, mit2704@gmail.com
²Ural State Forest Engineering University, ul. Sibirskiy Trakt, 37, Yekaterinburg, 620100, Russian Federation; labbav@yandex.ru

Keywords

Pinus sylvestris, carotenoids, flavonols, catechins, ascorbic acid, peroxidase activity, climate warming, antioxidant system

Funding

This work was financially supported by the Comprehensive Program of the Ural Branch of the Russian Academy of Sciences for 2018–2020 (Grant No. 18-4-4-10) and within the state assignment of the Federal State Budgetary Institution of Science “Institute Botanic Garden of the Ural Branch of the Russian Academy of Sciences”.
Acknowledgments: The authors are grateful to G.N. Novoselova, the leading engineer of the Ural State Forest Engineering University, for her direct participation in setting tasks for the research and conducting the laboratory analysis, as well as to the Nizhnevartovsk branch of the Federal State Budgetary Institution "Center for Laboratory Analysis and Technical Measurements in the Ural Federal District" of the Khanty-Mansiysk Autonomous Okrug (UGRA) for providing the results of the chemical and analytical work.

For citation

Shavnin S.A., Yusupov I.A., Montile A.A., Golikov D.Yu., Marina N.V. Seasonal Dynamics of Content of Antioxidant System Components in Needles of Scots Pine (Pinus sylvestris L.) Trees Situated in the Local Warming Impact Zone. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 2, pp. 38–57. (In Russ.). https://doi.org/10.37482/0536-1036-2023-2-38-57

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