Bioconversion of the Cellulose-containing Materials in the Arctic Region Conditions

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UDС

504.064.2

DOI:

10.17238/issn0536- 1036.2019.4.179

Abstract

The regions of the Far North and the Arctic (the territory of which includes the Arkhangelsk region) are characterized by extreme natural and climatic factors: low temperatures in winter, a large daily temperature drop, frequent snowstorms in winter and rains in summer. These conditions significantly affect the enzymatic activity of soil microorganisms. The aim of the work is to assess the effect of the enzymatic treatment temperature on the initial stages of biodegradation of the cellulose – containing materials. The study used samples of cardboard and paper products: newspaper paper with color printing, container cardboard and white office paper. Bacterial cellulose was obtained by cultivating a symbiosis of the genus Acetobacter bacteria and yeast on a glucose medium in static conditions at 25 °C. to simulate biodegradation, a laboratory enzyme preparation was used, produced by a strain of a microscopic fungus. The activity of soil cellulases initiating bioconversion of cellulose-containing materials in soils of municipal solid waste storage areas of the Arkhangelsk region was low and amounted to less than 10 μg of glucose/10 g of soil per 48 hours of exposure. The determining factor for the bioconversion process in the Arctic region is the soil temperature. It is shown that while a decrease in temperature to 5...15 °C, the bioconversion rate of cellulose-containing materials of plant origin is reduced by 2...8 times compared to the temperature of 30...50 °C, which is optimal for many enzymatic processes. It was found that bioconversion of bacterial cellulose to glucose is weakly dependent on the temperature of enzymatic treatment in the range of 5...15 °C and averages 20% for 1 day.

Authors

K.S. Bolotova¹, Candidate of Engineering, Assoc. Prof.; ResearcherID: G-1760-2019, ORCID: 0000-0002-7916-2410
O.V. Travina², Intern-researcher; ResearcherID: T-2952-2018, ORCID: 0000-0001-7049-9271
A.S. Aksenov¹, Candidate of Engineering, Head of the department; ResearcherID: C-7289-2015, ORCID: 0000-0003-1013-1357
M.V. Emelyanova¹, Candidate of Engineering, Assoc. Prof.; ORCID: 0000-0001-6600-6526
V.A. Rudakova¹, Candidate of Engineering, Assoc. Prof.; ORCID: 0000-0002-3447-4226
A.V. Kanarskiy³, Doctor of Engineering, Prof.; ResearcherID: O-8113-2016, ORCID: 0000-0002-3541-2588

Affiliation

¹Northern (Arctic) Federal University named after M.V. Lomonosov, 17 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163002, Russian Federation; e-mail: k.bolotova@narfu.ru, a.s.aksenov@narfu.ru, m.emelyanova@narfu.ru, v.rudakova@narfu.ru
²Federal State Budgetary Institution of Science of the Federal Research Centre for Arctic Integrated Research named after academician N.P. Laverov, Russian Academy of Sciences, 23 Naberezhnaya Severnoy Dviny, Arkhangelsk, 163000, Russian Federation; e-mail: travina.oksana136@yandex.ru
³Kazan National Research Technological University, 68 K. Marx str., Kazan, 420015, Russian Federation; e-mail: alb46@mail.ru

Keywords

biodegradation of cellulose-containing materials, bacterial cellulose, enzymatic hydrolysis, cellulose

Funding

The research was financed by the RFBR within the framework of the scientific project № 18-33-00855 “The supramolecular organization of the cellulose microfibrils of phytogenous and bacterial origin” hardware-assisted by the “Arctic” SC of the Northern (Arctic) Federal University named after M.V. Lomonosov, with the use of enzymatic agent provided by the Institute of biochemistry named after A.N. Bach.
*The article was published in the framework of implementation the development program of scientific journals in 2019

For citation

Bolotova K.S., Travina O.V., Aksenov A.S., Emelyanova M.V., Rudakova V.A., Kanarskiy A.V. Bioconversion of Cellulose-containing Materials in the Arctic Region Conditions. Lesnoy Zhurnal [Forestry Journal], 2019, no. 4, pp. 179–186. DOI: 10.17238/issn0536- 1036.2019.4.179

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Received on September 19, 2018