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A.S. Aksenov, N.A. Kuz’mina Complete text of the article:Download article (pdf, 0.4MB )UDС579.64:633.878.32DOI:10.17238/issn0536-1036.2018.4.161AbstractBiotechnologies provide an opportunity to solve global problems of sustainable development of economic activity. The study of biodiversity of plant endophytic bacteria allows creating new effective biologics for adaptive plant growing. The goal of research is to identify endophyte poplar bacteria in the North (Arkhangelsk). Samples were selected at the beginning of October in the crowned trees of laurel-leafed poplar and fragrant poplar, which had no external signs of pathology and stem rot. The studies were based on the characteristics of isolates obtained from the internal parts of the central wood of poplar tree stems after surface sterilization. Sawdust was examined by the fouling method. Meatpeptone agar was used as the nutrient medium. To determine the biochemical activity of pure bacterial cultures, Hiss's differential diagnostic media were used, including maltose, glucose, sucrose, sorbitol, mannitol, and lactose. For bacterial isolates, identification was carried out by sequencing fragments of the 16S рРНК gene and comparing the resulting nucleotide sequences with those homologous from the NCBI's GenBank database. When the sawdust was sowed in the nutrient medium, fouling with bacterial mass was noted. The sawdust of laurel-leafed poplar overgrew more intensively in comparison with fragrant poplar. Four strains of bacteria (Bacillus pumilus, Lysinibacillus fusiformis, Staphylococcus pasteuri, Brenneria salicis) were isolated from the laurel-leafed poplar wood, and 2 strains were isolated from fragrant poplar (Bacillus safensis, Brenneria populi). The article presents the morphological and biochemical characteristics of bacteria. Most bacteria successfully use the carbon components of the Hiss’ media, with the exception of sorbitol. The ability to metabolize lactose and mannitol is particularly unstable. The widest range of carbohydrates is fermented by strains belonging to the genus Brenneria.AuthorsA.S. Aksenov1, Candidate of Engineering Sciences, Associate ProfessorN.A. Kuz’mina2, Research Assistant Affiliation1Northern (Arctic) Federal University named after M.V. Lomonosov, Naberezhnaya Severnoy Dviny, 17, Arkhangelsk, 163002, Russian Federation; e-mail: a.s.aksenov@narfu.ru2Institute of Biogeography and Genetic Resources, N. Laverov Federal Center for Integrated Arctic Research, Russian Academy of Sciences, Naberezhnaya Severnoy Dviny, 23, Arkhangelsk, 163000, Russian Federation Keywordsbacterial endophyte, laurel-leafed poplar, fragrant poplar, woodFor citationAksenov A.S., Kuz’mina N.A. Bacterial Endophytes of Poplar. Lesnoy zhurnal [Forestry journal], 2018, no. 4, pp. 161–166. DOI: 10.17238/issn0536-1036.2018.4.161References1. Blagova D.K., Sarvarova E.R., Khayrullin R.M. Vydelenie i kharakteristika bakterial’nykh endofitov morkovi (Daucus carota L. VAR. SATIVUS) [Isolation and Characterization of Bacterial Endophytes from Carrots (Daucus carota L. VAR. SATIVUS)]. Vestnik Orenburgskogo gosudarstvennogo universiteta [Vestnik of the Orenburg State University], 2014, no. 13(174), pp. 10–12.2. Lyzhin D.N. Perspektivy razvitiya bioekonomiki v usloviyakh globalizatsii [Prospects of Bioeconomic Development in the Context of Globalization]. Problemy natsional’noy strategii [National Strategy Issues], 2014, no. 2(23), pp. 79–94. 3. Maksimov I.V., Abizgil’dina R.R., Pusenkova L.I. Stimuliruyushchie rost rasteniy mikroorganizmy kak al’ternativa khimicheskim sredstvam zashchity ot patogenov (obzor) [Plant Growth Promoting Rhizobacteria as Alternative to Chemical Crop Protectors from Pathogens (Review)]. Prikladnaya biokhimiya i mikrobiologiya [Applied Biochemistry and Microbiology], 2011, vol. 47, no. 4, pp. 373–385. 4. Shekhovtsоva N.V., Marakaev O.A., Pervushina K.A., Kholmogorov S.V., Tsaplyaeva K.G. Obrazovanie auksinov endofitnymi bakteriyami podzemnykh organov Dactylorhiza maculata (L.) SOO (ORCHIDACEAE) [Auxine Production by Endophytic Bacteria from Underground Organs of Dactylorhiza maculata (L.) SOO (ORCHIDACEAE)]. Vestnik Orenburgskogo gosudarstvennogo universiteta [Vestnik of the Orenburg State University], 2011, no. 12(131), pp. 366–368. 5. Shcherbakov A.V., Bragina A.V., Kuz’mina E.Yu., Berg K., Muntyan A.N., Makarova N.M., Mal’fanova N.V., Kardinale M., Berg G., Chebotar’ V.K., Tikhonovich I.A. Endofitnye bakterii sfagnovykh mkhov kak perspektivnye ob”ekty sel’skokhozyaystvennoy mikrobiologii [Endophytic Bacteria of Sphagnum Mosses as Promising Objects of Agricultural Microbiology]. Mikrobiologiya [Microbiology], 2013, vol. 82, no. 3, pp. 312–322. 6. Barac T., Weyens N., Oeyen L., Taghavi S., van der Lelie D., Dubin D., Spliet M., Vangronsveld J. Field Note: Hydraulic Containment of a Btex Plume Using Poplar Trees. International Journal of Phytoremediation, 2009, vol. 11, iss. 5, pp. 416–424. 7. Khan Z., Guelich G., Phan H., Redman R., Doty S. Bacterial and Yeast Endophytes from Poplar and Willow Promote Growth in Crop Plants and Grasses. International Scholarly Research Network. ISRN Agronomy, 2012, vol. 2012. 11 p. doi: 10.5402/2012/890280 8. Moore F.P., Barac T., Borremans B., Oeyen L., Vangronsveld J., van der Lelie D., Campbell C.D., Moore E.R.B. Endophytic Bacterial Diversity in Poplar Trees Growing on a BTEX-Contaminated Site: the Characterization of Isolates with Potential to Enhance Phytoremediation. Systematic and Applied Microbiology, 2006, vol. 29, iss. 7, pp. 539–556. 9. Posada F., Vega F.E. Establishment of the Fungal Entomopathogen Beauveria bassiana (Ascomycota: Hypocreales) as an Endophyte in Cocoa Seedlings (Theobroma cacao). Mycologia, 2005, vol. 97, iss. 6, pp. 1195–1200. doi: 10.3852/mycologia.97.6.1195 10. Strobel G., Daisy B., Castillo U., Harper J. Natural Products from Endophytic Microorganisms. Journal of Natural Products, 2004, vol. 67, iss. 2, pp. 257–268. doi: 10.1021/np030397. 11. Taghavi S., Garafola C., Monchy S., Newman L., Hoffman A., Weyens N., Barac T., Vangronsveld J., van der Lelie D. Genome Survey and Characterization of Endophytic Bacteria Exhibiting a Beneficial Effect on Growth and Development of Poplar Trees. Applied and Environmental Microbiology, 2009, vol. 75, iss. 3, pp. 748–757. 12. Ulrich K., Ulrich A., Ewald D. Diversity of Endophytic Bacterial Communities in Poplar Grown Under Field Conditions. FEMS Microbiology Ecology, 2008, vol. 63, iss. 2, pp. 169–180. 13. van der Lelie D., Taghavi S., Monchy S., Schwender J., Miller L., Ferrieri R., Rogers A., Wu X., Zhu W., Weyens N., Vangronsveld J., Newman L. Poplar and its Bacterial Endophytes: Coexistence and Harmony. Plant Science, 2009, vol. 28, iss. 5, pp. 346–358.
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