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Influence of Chronic Ionizing Radiation on the Variability of Mitotic Activity of Pinus sylvestris L. Tissues

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A.V. Skok, V.N. Sorokopudov, I.N. Glazun

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The development of nuclear power engineering will increase in the future, due to the continued likelihood of radionuclides entering the environment. The relevance of studying the genetic material of conifers as a reliable bioindicator was revealed. It is known that under the influence of ionizing radiation there is an accumulation of genetic abnormalities in pine (Pinus sylvestris L.) progeny. The stages of gametogenesis, zygotes and young seedlings of plant organisms are most sensitive to ionizing radiation. It is important to determine the variability of the mitotic index (MI) of tissues of Scots pine (Pinus sylvestris L.) progeny in areas with different levels of ionizing radiation, including in the exclusion zone. The exposure dose rate was measured at the soil level and 1 m from the ground surface. Germination of seeds was carried out in a thermostat on wet filter paper. Roots of seedlings 0.5–1 cm long were fixed in a mixture of 96 % ethanol and glacial acetic acid. Root staining was carried out in a solution of acetocarmine. Tissue softening was carried out with a strong chloral hydrate solution. The total number of dividing cells, as well as pathological mitoses (PM) were counted on squash preparations using a microscope. The mitotic index and the duration of the phases of mitosis were determined. An increase in the level of radioactive contamination increases the cell division rate in prophase, anaphase, and telophase, but decreases the duration of metaphase, and also changes the relative duration of mitosis phases. With an increase in the exposure dose rate, the number of pathological mitoses naturally increases. The spectrum of mitosis disorders is represented by various abnormalities of the chromosome apparatus in anaphase: exit and lagging of chromosomes, bridges. Herewith, the number of anaphases with bridges increases significantly with simultaneous exit and lagging of chromosomes.


Anna V. Skok1, Candidate of Biology, Assoc. Prof.;
Vladimir N. Sorokopudov2, Doctor of Agriculture, Prof.; ResearcherID: B-1520-2018,
Igor’ N. Glazun1, Candidate of Agriculture, Assoc. Prof.; ResearcherID: ABC-9610-2020,


1Bryansk State Engineering and Technology University, prosp. Stanke Dimitrova, 3, Bryansk, 241037, Russian Federation; e-mail:,
2Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, ul. Timiryazevskaya, 49, Moscow, 127550, Russian Federation; e-mail:


Pinus sylvestris L., exposure dose rate, mitosis phases, anaphase abnormalities, bridges, chromosome lagging, chromosome exit

For citation

Skok A.V., Sorokopudov V.N., Glazun I.N. Influence of Chronic Ionizing Radiation on the Variability of Mitotic Activity of Pinus sylvestris L. Tissues. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 1, pp. 112–119. DOI: 10.37482/0536-1036-2021-1-112-119


1. Abramov V.I., Dineva S.V., Rubanovich A.V., Shevchenko V.A. Genetic Consequences of Radioactive Contamination of Populations of Arabidopsis thaliana Growing in the 30-km Zone of the Chernobyl Accident. Radiatsionnaya biologiya. Radioekologiya [Radiation biology. Radioecology], 1995, vol. 35, iss. 5, pp. 676–689.
2. Butorina A.K., Vostrikova T.V. The Study of Cytogenetic Parameters in White Birch under Anthropogenic Stress. Integration of Science and Higher Forestry Education in Forest and Forest Product Quality Management: Proceedings of the International Scientific and Practical Conference (Voronezh, September 25–27, 2001). Voronezh, 2001, pp. 78–82.
3. Butorina A.K., Kalaev V.N., Mironov A.N., Smorodinova V.A., Mazurova I.E., Doroshev S.A., Sen’kevich E.V. Cytogenetic Variation in Populations of Scots Pine. Ekologiya [Russian Journal of Ecology], 2001, no. 3, pp. 216–220. DOI: 10.1023/A:1011366328809
4. Goryachkina O.V., Sizykh O.A. Cytogenetic Responses of Coniferous Plants in Anthropogenically Disturbed Areas of Krasnoyarsk and Its Vicinities. Hvojnye boreal’noj zony [Conifers of the boreal area], 2012, vol. 30, no. 1-2, pp. 46–51.
5. Grodzinskiy D.M. Radiobiology of Plants. Kiev, Naukova Dumka Publ., 1989. 380 p.
6. Dmitriyeva S.A. Methodology of Environmental Biotesting Based on Cytogenetic Studies. Proceedings of the 5th International Conference on Karyology, Karyosystematics and Molecular Systematics of Plants. Saint Petersburg, 2005, pp. 31–32.
7. Zaytsev G.N. Mathematical Statistics in Experimental Botany. Moscow, Nauka Publ., 1984. 424 p.
8. Ipat’yev V.A., Baginskiy V.F., Bulavik I.M., Dvornik A.M., Volchkov V.E., Goncharenko G.G. Forest. Man. Chernobyl. Forest Ecosystems after the Chernobyl Accident: State, Forecast, Reaction of the Population, Ways of Rehabilitation. Ed. by V.A. Ipat’yev. Gomel, Institut lesa NAN Belarusi Publ., 1999, pp. 55–67.
9. Kalashnik N.A. Chromosome Aberrations as Indicator of Technogenic Impact on Conifer Stands. Ekologiya [Russian Journal of Ecology], 2008, no. 4, pp. 276–286. DOI: 10.1134/S106741360804005X
10. Krivolutskiy D.A., Tikhomirov F.A., Fedorov E.A. The Effect of Ionizing Radiation on Biogeocenosis. Moscow, Nauka Publ., 1988. 240 p.
11. Muratova E.N. Chromosome Numbers of Gymnosperms: Pinaceae (Picea-Pinus). Botanicheskii Zhurnal, 2011, vol. 96, no. 10, pp. 1389–1404.
12. Pozolotina V.N. Long-Term Effects of Radiation on Plants: Dr. Biol. Sci. Diss. Yekaterinburg, 2001. 401 p.
13. Alberts B., Johnson A., Lewis J., Raff M., Roberts K., Walter P. Molecular Biology of the Cell. New York, Garland Science, 2008. 1616 p.
14. Evert R.F., Eichhorn S.E. Raven Biology of Plants. W.H. Freeman & Co, 2013. 900 p.
15. Hartwell L., Hood L., Goldberg M., Reynolds A.E., Silver L. Genetics: From Genes to Genomes. McGraw-Hill Education, 2010. 816 p.
16. Lackie J.M. The Dictionary of Cell and Molecular Biology. Amsterdam, Academic Press, 2013. 750 p. DOI: 10.1016/C2009-0-64239-2
17. Morgan D.O. The Cell Cycle: Principles of Control. London, New Science Press, 2007. 297 p.
18. Redei G.P. Encyclopedia of Genetics, Genomics, Proteomics, and Informatics. The Netherlands, Springer, 2008. 2201 p.
19. Rodionov S.S. Environmental Management in the Present State of the Information. The Strategies of Modern Science Development: Proceedings of the III International Conference. Yelm, WA, Science Book Publishing House, 2013, pp.183–186.
20. Ziegler I. The Effect of SO2 Pollution on Plants Metabolism. Residue Reviews. New York, Springer-Verlag, 1975, vol. 56, pp. 79–105. DOI: 10.1007/978-1-4613-9388-7_2

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