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Starch Content in Shoot Tissues of Different Spruce Species (Picea A. Dietr.) in Introduction

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N.N. Besschetnova, V.P. Besschetnov, A.V. Kul'kova,  I.V. Mishukova

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UDС 630*232.12:582.475.2




Introduction is one of the most effective methods of afterculture of local dendroflora with valuable plant species. To diagnose the success of plants introduction we should clarify the nature of their adaptation to the new growth conditions. The composition and the ratio of reserve constituents and metabolites in tissue cells are highly important for assessing the plant establishment. Starch is the most important reserve constituent in the development of woody plants. The increase of temperature significantly affects the content of this nutrient. The increase of the starch content of tissue cells is associated with the release of plants from quiescence and preparation for the growing season. We have investigated the biological specificity of 13 spruce species,introduced in the Nizhny Novgorod region, and have established their heterogeneity in the starch content in tissue cells of the annual shoots, which could have the adaptation significance. Determination of starch in tissue cells of the annual shoots was detected by the Lugol staining reaction. Sections after staining with appropriate reagents and fixation were analyzed using a micrometer “Mikmed-2”. The estimation of the content of reserve constituents on the transverse section of the shoot was given for each accounting area separately and in the score in all accounting areas. Statistical and dispersion analyses were performed according to the common methods. We calculated the mean values for each botanical species included in the analysis scheme. The ratio between the species of the genus Spruce by the starch content in different tissues was not the same. The differences between the exotic species appeared under the same environmental conditions. The variance analysis confirmed the significance of differences between the studied spruce species in the starch content.


N.N. Besschetnova1, Doctor of Agricultural Sciences, Associate Professor V.P. Besschetnov1, Doctor of Biological Sciences, Professor A.V. Kul'kova1, Postgraduate Student I.V. Mishukova2, Leading Biologist


1State agricultural academy of Nizhny Novgorod, pr. Gagarina, 97, Nizhny Novgorod, 603107, Russian Federation; e-mail:
2Botanical Garden of the N.I. Lobachevsky State University of Nizhny Novgorod, ul. Botanicheskiy Sad, 1, Nizhny Novgorod, 603062, Russian Federation; e-mail:


spruce, introduction, adaptation, resistance, starch, shoot tissue.

For citation

Besschetnova N.N., Besschetnov V.P., Kul'kova A.V., Mishukova I.V. Starch Content in Shoot Tissues of Different Spruce Species (Picea A. Dietr.) in Introduction. Le-snoy zhurnal [Forestry journal], 2017, no. 4, pp. 57–68. 10.17238/issn0536-1036.2017.4.57


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Received on March 19, 2017

Starch Content in Shoot Tissues of Different Spruce Species (Picea A. Dietr.) in Introduction


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