Fraud Warning
We officially declare that NO MONEY from authors and members of the Editorial Board IS CHARGED! A big request to ignore spam e-mails.

Address: 17 Naberezhnaya Severnoy Dviny, Arkhangelsk 163002 Russian Federation. Northern (Arctic) Federal University named after M.V.Lomonosov. Office 1425

Phone / Fax: (818-2) 21-61-18
E-mail: forest@narfu.ru
http://lesnoizhurnal.ru/en/

Archive

Pollination in Common Juniper (Juniperus communis L.): Involving of Pollen and the Male Gametophyte Influence on the Ovules and “Cypress Cones” Development

Версия для печати
Creative Commons License
These works are licensed under a Creative Commons Attribution 4.0 International License.

M.V. Surso, N.V. Selivanova

Complete text of the article:

Download article (pdf, 0.9MB )

UDС

UDC 582.477.6:581.162.3

DOI:

10.17238/issn0536-1036.2016.4.40

Annotation

"By the light microscopy we studied the development of the pollinated and unpollinated ov-ules of juniper since the receptation period prior to the “cypress cones” formation. The mor-phogenesis of unpollinated ovules and macrostrobiles proceeded without the essential viola-tions prior to the beginning of the cellular gametophyte formation. Soon after the beginning of its formation there was a gradual attenuation of mitoses in the unpollinated ovules, the signs of tissue degeneration in the chalazae and archegonial complexes zones. At the same time the safety of unpollinated macrostrobiles remained high. To the beginning of a late embryogenesis almost all unpollinated female cones in the pollinated ovules were lost. The remaining single unpollinated cones showed the signs of degeneration of external protective tissue and parenchyma of the “cypress cones”. The dynamics of exudation and retraction of a pollination drop was studied in vitro in order to learn the influence of the external agents on their dynamics in juniper. 3 variants of pollination were tested: by vital pollen of juniper (a collection of the previous year); by freshly pollen of Scotch pine; without pollination. At pollination by foreign pollen the retraction delayed for at least 12 hours. In the absence of pollination the exudation activity of a micropylar zone of the ovules lasted at least 60 hours. Full retraction of the secretory fluids at the same time took place after 120 hours. The quali-tative composition of volatile fractions of organic compounds of a juniper pollination drop was studied by the gas chromatograph/mass spectrometer QP-2010 Ultra (“Shimadzu”, Japan). The amino acid composition of the juniper exudation secretion and pollen was de-fined by the aminoacid analyzer BioChrom 30+ (“Biochrom”, Great Britain). Sugar compo-sition of the juniper pollination drop and pollen was studied by a HPLC system Nexera XR (“Shimadzu”, Japan). More than 40 volatile substances belonging to the various classes of
organic compounds were identified. Their ratio is the next: alkanes – 33.79 %, monoter-penes – 0.29 %, triterpenes – 16.97 %, sesquiterpene alcohols – 1.33 %, esters – 18.41 %, carboxylic acids – 1.81 %, carboxazylic acids – 8.81 %. The results of the research confirm a complex chemical composition and multifunctional performance of a pollination drop. The pollination mechanism in juniper is effective and selective. The lack of pollination leads to a destructive type of the ovules and “cypress cones” tissue development."

Authors

M.V. Surso, Doctor of Agricultural Sciences, Senior Researcher N.V. Selivanova, Candidate of Chemical Sciences, Senior Researcher

Authors job

Institute of Ecological Problems of the North, Ural Branch of the Russian Academy of Sciences, Naberezhnaya Severnoy Dviny, 23, Arkhangelsk, 163000, Russian Federation; e-mail: surso@iepn.ru

Keywords

common juniper, pollination, pollination drop, ovule.

References


1. Jensen W.A. Botanical Histochemistry. Principles and Practices. USA, San Fran-cisco, 1962. 408 p.
2. Pausheva Z.P. Praktikum po tsitologii rasteniy [Handbook on Plants Cytology]. Moscow, 1970. 255 p.
3. Ruguzova A.I. Morfo-fiziologicheskie osobennosti formirovaniya pyl'tsevykh zeren i opyleniya u nekotorykh vidov sem. Cupressaceae [Morphological and Physiological Characteristics of the Pollen Grains Formation and Pollination in Some Species of the Cu-pressaceae Family]. Botanicheskiy zhurnal, 2004, vol. 87, no. 7, pp. 1111–1121.
4. Ruguzov I.A., Zakharenko G.S., Sklonnaya L.U. Zakonomernosti opyleniya ras-teniy poryadka Coniferales [Laws of Plant Pollination of the Coniferales]. Tsitologo-embriol. i genetiko-biokhim. osnovy opyleniya i oplodotvoreniya rasteniy [Cytological and Embriological and Genetic and Biochemical Basics of Pollination and Fertilization of Plants]. Kiev, 1982, pp. 257–259.
5. Ruguzov I.A., Sklonnaya L.U., Chebotar' A.A. Ob opylitel'noy kaple u khvoynykh [On the Pollination Drop in Conifers]. Botanicheskiy zhurnal, 1992, vol. 77, no. 12, pp. 40–52.
6. Coulter A., Poulis A.D., von Aderkas P. Pollination Drop as Dynamic Apoplastic Secretions. Flora, 2012, vol. 207, pp. 482–490.
7. Doyle J. Developmental Lines in Pollination Mechanisms in the Coniferales. Sci. Proc., 1945, vol. 24, no. 5, pp. 43–62.
8. Dumas C., Bowman R.B., Gaude T., Guilly C.M., Heizmann Ph., Roeckel P., Rougier M. Stigma and Stigmatic Secretion Reexamined. Phyton, 1988, vol. 28, no. 2, pp. 193–200.
9. Gelbart G., von Aderkas P. Ovular Secretions as Part of Pollination Mechanisms in Conifers. Annals of Forest Science, 2002, vol. 59, pp. 345– 357.
10. Mugnaini S., Massimo N., Massimo G., Beti P., Ettore P. Pollination Drop Withdrawal in Juniperus communis: Response to Biotic and Abiotic Particles. Caryologia, 2007, vol. 60, no. 1–2, pp. 182–184.
11. Nepi M., von Aderkas P., Wagner R., Mugnaini S., Coulter A., Pacini E. Nectar and Pollination Drops: How Different are They? Annals of Botany, 2009, vol. 104, pp. 205–219.
12. O'Leary S.J.B. Proteins in the Ovules Secretions of Conifers: Ph. D. Diss. Cana-da, Antigonish, Nova Scotia, 1998. 190 p.
13. Owens J.N., Takaso T., Runions C.J. Pollination in Conifers. Trends Plant Sci., 1998, vol. 3, pp. 479–485.
14. Poulis B.A.D., O?Leary J.B., Haddow J.D., von Aderkas P. Identification of Pro-teins Present in the Douglas Fir Ovular Secretion: an Insight into Conifer Pollen Selection and Development. Intern. J. Plant Sci., 2005, vol. 166, pp. 733–739.
15. Wagner R.E., Mugnaini S., Sniezko R., Hardie D., Poulis B., Nepi M., Pacini E., von Aderkas P. Proteomic Evaluation of Gymnosperm Pollination Drop Proteins Indicates Highly Conserved and Complex Biological Functions. Sex Plant Reprod., 2007, vol. 20, pp. 181–189.

Pollination in Common Juniper (Juniperus communis L.): Involving of Pollen and the Male Gametophyte Influence on the Ovules and “Cypress Cones” Development

 

INDEXED IN: