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Lesnoy Zhurnal

Evaluation of the Thermostability of English Oak and Rock Oak and Their Degree of Adaptation to the Effects of Heat Shock

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P.A. Cuza

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The leaves of sessile oak (Quercuspetraea Liebl.) and pedunculate oak (Quercusrobur L.) were subjected to heat shock at various high temperatures. The damage caused by the heat shock to the cellular structures of the leaves was determined using the electrolyte leakage technique. In the investigated species, a sigmoidal increase of electrolyte leakage from leaf tissues, depending of the applied temperatures, was observed. Pedunculate oak leaves, as compared with sessile oak ones, have shown increased resistance to high temperatures, suggesting that heat tolerance in pedunculate oak is higher than in sessile oak. Experiments with fractionation of heat shock doses allowed the estimation of the influence of the first dose value on induction of the lives adaptive capacity of the sessile oak leaves during different periods of time after their application. If the first fraction of dose was moderate, the thermotolerance of leaves grew rapidly. So, the functional status of leaves depended on three components that characterized the fractionation of dose: the value of the first part of dose (1), the value of the second part of dose two (2), the duration of the period that has passed between two fractions of dose (3). Summary effect of fractionated dose of heat shock is the result of balance between processes of degradation, recovery of damages, and adaptation. After application of moderate fractions of heat shock dose. the processes of the induction of adaptation dominated. Because of this the thermotolerance of leaves after application of the first dose of heat shock increased. After the application of higher fractions of dose, the processes of degradation prevailed under those of the recovery and adaptation. In combination they lead to the reduction leaves thermotolerance. The obtained results suggest that the method of fractional heat shock doses make possible determination of the initial thermotolerance and the adaptive capacity of leaves. Pooling of the processes that determine the initial leaves thermotolerance and their adaptive potential under variation of seasonal temperatures is important for plants survival in arid conditions.


P. A. Cuza, Doctor of Biol. Sciences, prof.

Authors job

State University of Moldova, 60 A.Mateevici St.,Chisinau, Republic of Moldova; e-mail:


Quercus robur L., Q. petraea Liebl., thermotolerance, leaves, heat shock

For citation

Cuza P. A. Evaluation of the thermostability of english oak and rock oak and their degree of adaptation to the effects of heat shock. Lesnoy Zhurnal [Forestry Journal], 2019, no. 4, pp. 187–199. DOI: 10.17238/issn0536-1036.2019.4.187


1. Alexandrov V.Ya. Reactivity of Cells and Proteins. Leningrad, Nauka Publ., 1985. 318 p.
2. Alexandrov V.Ya., Kislyuk I.M. Reaction of Cells to Heat Shock: Physiological Aspect. Cytology. 1994, vol. 3, no.1, pp. 5–59.
3. Gorban I.S. Increase of Heat Resistance and Stimulation of the Reparative Ability of Plant Cells After Reversible Heat Damage, Determined by the Change in the Viscosity of the Protoplasm. Cytology. 1983, vol. 25, no. 1. pp. 64–71.
4. Enkova E.I. Tellerman Forest and Its Restoration. Voronezh, Voronezh University Publ. 1976. 214 p.
5. Titov A.F., Akimova, T.V., Talanova V.V., Topchiyeva L.V. Resistance of Plants in the Initial Period of Unfavourable Temperatures: Monography. Moscow, Nauka Publ., 2006. 143 p.
6. Alexandrov V.Ya., Lomagin A.G., Feidman N.L. The Responsive Increase in Thermostability of Plant Cells. Protoplasma. 1970, vol. 69, pp. 417–458.
7. Anderson J.T., Willis J.H., Mitchell-Olds T. Evolutionary Genetics of Plant Adaptation. Trends in Genetics, 2011, vol. 27 (7), pp. 258–266.
8. Cabral R., O’Reilly C. The Physiological Responses of Oak Seedlings to Warm Storage. Can. Jour. For. Res., 2005, vol. 35. no. 10, pp. 2413–2422.
9. Cuza P. Apreciere a Rezistenţe Istejarului Pufos (Quercus Pubescens Willd.) Şistejarul Uipedunculat (Quercusrobur L.) La Acţiunea Temperaturilor Înalte. Buletinul Academiei de Ştiinţe a Moldovei. Ştiinţele Vieţii, 2008, no. 3 (306), pp. 48–56.
10. Cuza P. Capacitatea de Adaptare a Frunzelor Stejaruluipufos (Quercus Pubescens Willd.) in Funcţie de Doză şi Durata Fracţionări Idozelor Şocului Termic. Mediulambiant, 2008, no. 6 (42), pp. 23–26.
11. Cuza P. Instalarea şi Menţinerea Speciilor de Stejar (Aspecte Tteoretice şi Practice). Chişinău, Mediul Ambiant Publ. 2017. 246 p.
12. Dascaliuc Al., Cuza P. Specificul adaptării frunzelor stejarului pedunculat (Quercus Robur L.) la Şocul Termic în Funcţie de Valoarea Temperaturii şi Durata de Acţiune. Mediul ambient, 2008, no. 3 (39), pp. 34–37.
13. Dascaliuc Al., Cuza P. Capacitatea de Adaptare a Aparatului Fotosintetic al Speciilor de stejar (Quercus Robur, Q. Petraea, Q. Pubescens) la Acţiunea Temperaturilor Înalte. Mediulambiant, 2011, no. 2 (56), pp. 33–36.
14. Dascaliuc A., Tate R. Systemic in Determining the Biological Role of Natural Products. Tehnologii Biologice Avansate şi Impactul lor în Economie. Produse Naturale: Tehnologii de Valorificare a lor în Agricultură, Medicină şi Industria Alimentară: Mater. Simpoz. al 2-lea. Chişinău, 2005. pp. 24–37.
15. Dascaliuc A., Ivanova R., Arpentin Gh. Systemic Approach in Determining the Role of Bioactive Compounds. Advanced Bioactive Compounds Countering the Effects of Radiological, Chemical and Biological Agents. Strategies to Counter Biological Damage. NATO Science for Peace and Security. Series A. Chemistry and Biology. Springer, 2013, pp. 121–131.
16. Ingram D.L Modeling High Temperature and Exposure Time Interactions on Pittosporum Tobira Root Cell Membrane Thermostability. J. Amer. Soc. Hort. Sci., 1985, vol. 110. no. 4, pp. 470–473.
17. Kuster T.M., Bleuler P., Arend M., Günthardt-Goerg M.S., Schulin R. Soil Water, Temperature Regime and Growth of Young Oak Stands Grown in Lysimeters Subjected to Drought Stress and Air Warming. Bulletin BGS, 2011, vol. 32, pp. 7–12.
18. Larkindale J., Hall J.D., Knight M.R., Vierling E. Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance. Plant Physiol, 2005, vol. 138, pp. 882–897.
19. Levitt J. Responses of Plants to Environmental Stresses. New York, Academic Press Publ., 1980, vol. 1. 568 p.
20. Martineau J.R., Specht J.E., Williams J.H., Sullivan C.Y. Temperature Tolerance in Soybeans. I. Evaluation of a Technique for Assessing Cellular Membrane Thermostability. Crop Science, 1979, vol. 19, pp. 75–78.
21. Mortazavi M., O’Reilly C., Keane M. Stress Resistance Levels Change Little During Dormancy in Ash, Sessile Oak and Sycamore Seedlings. Ann. For. Sci., 2004, vol. 61, pp. 455–463.
22. Santarius K.A., Müller M. Investigation on Heat Resistance of Spinach Leaves. / Planta, 1979, vol. 146, pp. 529–538.
23. Stocker O. Morphologische und Physiologische der Dürreresistenz. Bern: Kali-Inst., 1958, pp. 79–93.
24. Sullivan C.Y. Mechanisms of Heat and Drought Resistance in Grain Sorghum and Methods of Measurement. // N.G. Rao and L.R. House (eds.). Sorghum in the Seventies. Oxford & I.B.H., New Delhi, 1972, India, pp. 267–274.
25. Suzuki N., Mittler R. Reactive Oxygen Species and Temperature Stresses: A Delicate Balance Between Signaling and Destruction. Physiologia Plantarum, 2006, vol. 126, pp. 45–51.

Received on April 14, 2019

Evaluation of the Thermostability of English Oak and Rock Oak and Their Degree of Adaptation to the Effects of Heat Shock